Hello Tahir:

Larvae  of Plodia interpunctella infected with granulosis virus stop feeding, become pale, flaccid, and very fragil, so that they often burst open releasing the viral content to the environment. Also infected larvae excret liquid excrements contaminating the food and rearing containers. To confirm if larvae are infected with granulosis virus, you need to isolate the virus from infected larvae. Macerete larvae in a solution of wáter, separate the virus by centrifugation of a homogenate of infected individuals. You can purify the virus diluting with 2% Sucrose and 0.1% Coomassie Blue Brillinat Blue R dye in double distilled wáter.  Preparations of purified virus consist of homogeneous populations of intact inclusion bodies (210 by 380 nm) whose buoyant density is 1.271 g/cm3 when centrifuged to equilibrium in sucrose gradients. Electron microscopy of thin-sectioned virus or of virus sequentially disrupted on electron microscope grids shows three components: protein matrix, envelope, and nucleocapsid.

Please check this paper by M. Boots and M. Begon, 1995. Strain diferences in the Indian Meal Moth Plodia interpunctella in response to a Granulosis Virus.

http://link.springer.com/article/10.1007%2FBF02515759#page-1

Also for the protocol of virus purification check this paper by K. Tweeten et. al. 1997. Isolation and purification of a granulosis virus from infected larvae of the Indian meal moth, Plodia interpunctella.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC242650/

Regards,

Luis Miguel

Dear Luis Miguel Constantino,

Thanks for your time for this detailed answer. It will be helpful for me. Thanks once again.