I have a request to measure IL2R on different cell lines by Flow Cytometry. Is this usually on the surface?
Dear Sandy Cole ,
You could probably start from collecting information on anti-CD25 (IL-2Rα).
For example for humans:
https://www.biolegend.com/en-us/products/pe-anti-human-cd25-antibody-616
And for mice:
https://www.biolegend.com/en-us/products/pe-anti-mouse-cd25-antibody-424
Regards,
Yes, after I sent this question, I looked up IL2R in BD's catalog and it looks like it's synonymous with CD25 so pretty straight-forward. Thank!
These research group did.
you may read their method.
Original Paper
Interleukin-2 expression in human carcinoma cell lines and its role in cell cycle progression
Torsten E Reichert, Shigeki Nagashima, […]Theresa L Whiteside
Oncogene volume 19, pages514–525(2000)
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