Does anyone know one antibody that works against Mycobacterium spp?

Anti-Mycobacterium antibody: does anyone know one antibody that works against Mycobacterium spp. (mainly for immunofluorescence)? Many thanks in advance! Cristiane

04 January 2021 9,724 3 View

Can likert-type statements formulation derive solely from literature review?

28 October 2020 9,951 3 View

How to report analytical uncertainty calculations in a research paper about environmental contamination?

We submitted a paper investigating trace elements levels in multiple abiotic and biotic matrices using analytical protocols already established. One of the reviewers required us to report...

27 October 2020 8,453 4 View

Does 2-methoxyethanol reacts with air?

Hello, I'm trying to use 2-methoxyethanol/Cellosolve to dilute methylumbelliferyl phosphate for phosphatase activity analysis. However, we never used this solvent before. It came with a sure/safe...

27 October 2020 7,302 1 View

Can we predict differential expression genes (MicroRNAs) by analyzing a DNA Methylation dataset?

29 July 2020 7,333 2 View

What is the best protocol for evaluate TMR-peptide?

I apologize if my question seems to naive, but I would like to do a protocol to determine bio-distribution of a peptide coupled to TMR in an animal model however, I am having some difficulties in...

07 July 2019 5,664 2 View

Anyone could give some tips for fish brain microdisection?

I want to isolate fish brain specific zones like telencephalon, cerebelum, opticum tectum. I am encoutering with the problem of hardness of tissue because in the moment we try to take fish brain...

30 April 2019 8,841 1 View

In which countries of the world have digital textbooks been used as a substitute for printed models?

I think the digital textbook is an important additional resource to the printed book for enabling portability and choices for the student.

21 March 2019 9,022 6 View

How can you read cel files using the R program?

27 February 2019 8,863 2 View

For electricity storage in buildings, can an equation relating cost ($) with size (MW) be established?

I am trying to come up with an equation for electricity storage in buildings, establishing a relationship between cost ($) and size/power (MW). Would it be merely a function of size (Cost = X *...

28 October 2018 5,255 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Is it possible to use maize mature seed to do transformation for maize transgenic study?

Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...

17 February 2021 1,434 13 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

Can any one explain why the ethyl ester Biodiesel (FAEE) has higher than that of the metyl ester FAME- both of them are derived from the same oil?

Biodiesels derived from waste vegetable oil transesterification with Ethanol (FAEE) show higher peroxide values than those derived by transesterification with methanol (FAME) prepared under the...

13 February 2021 193 6 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View