Hi everyone,

I'm part of a newly established virology lab working with Cytomegalovirus (HCMV). We're currently facing issues recovering HCMV from the BJ-5ta cell line. We're confident the virus infects the cells, as they become green due to the GFP-tagged virus, but when it comes to recovering and titering the virus, we consistently get negative results.

Has anyone else experienced a similar problem or have any suggestions?