Has anyone had success with an in-house von Willbrand Factor collagen binding assay (Type I)?

Zheila Azartash-Namin @Zheila_Azartash-Namin

11 November 2017 0 5K Report

Currently using vWF:CBA Collagen I 96/well kit in a research lab. They are pretty expensive, and sometimes have issues of unavailability. Has anyone had success with a recipe for an in-house assay?

Badges
Science topic

Similar topics
Microbiology
Assays

More Zheila Azartash-Namin's questions See All

How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for Metabarcoding?

I'm working on pollen DNA metabarcoding to identify the floral composition of honey. How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for the...

28 December 2020 9,132 1 View

Is there a method to recognize all species from a mixed PCR product?

Hi everybody, I used universal primers to find the origin of plant or plants made material by insect and now I have a mixed PCR product of several plants. Is there a method to recognize all...

29 November 2018 5,314 3 View

What is a good high molecular weight marker for evaluating vWF?

I'm a little new to this, so please forgive me. In electrophoresis and Western blotting to evaluate von Willebrand factor in agarose gel, what would be a good high MW marker?

09 October 2016 4,377 3 View

Can multiple freeze-thaw cycles be performed on a hydrogel (PVA/PVP) for crosslinking without changing the mechanical properties?

The polymer goes through 6 freeze-thaw cycles (21 hours at -19C and 3 hours at 25C). Does anyone know, if I perform 2 or 3 cycles in a day, would the polymer be the same?

03 April 2013 7,457 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View