Currently using vWF:CBA Collagen I 96/well kit in a research lab. They are pretty expensive, and sometimes have issues of unavailability. Has anyone had success with a recipe for an in-house assay?
I'm working on pollen DNA metabarcoding to identify the floral composition of honey. How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for the...
28 December 2020 9,459 1 View
Dear all; I have been trying to relax Tenorite (CuO) with monoclinic structure. During relaxation, the cell parameters change significantly and the final result is not consistent with the...
12 July 2020 559 2 View
Hi everybody, I used universal primers to find the origin of plant or plants made material by insect and now I have a mixed PCR product of several plants. Is there a method to recognize all...
29 November 2018 5,431 3 View
I'm a little new to this, so please forgive me. In electrophoresis and Western blotting to evaluate von Willebrand factor in agarose gel, what would be a good high MW marker?
09 October 2016 4,382 3 View
Dear all I want to fabricate the model by scale 1:100 and perform some wind tunnel tests to measure the velocity and pressure distribution on the model. Would you please guide me which scale would...
17 August 2014 4,267 6 View
The polymer goes through 6 freeze-thaw cycles (21 hours at -19C and 3 hours at 25C). Does anyone know, if I perform 2 or 3 cycles in a day, would the polymer be the same?
03 April 2013 7,472 1 View
I would like to know the pros/cons of using cross-linked PVA/PVP as an intervertebral disc, so I am curious to know if anyone knows of any negatives of using this in high stress, compressible, or...
17 March 2013 8,552 1 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View