18 March 2013 1 9K Report

I would like to know the pros/cons of using cross-linked PVA/PVP as an intervertebral disc, so I am curious to know if anyone knows of any negatives of using this in high stress, compressible, or other related applications.

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How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for Metabarcoding?

I'm working on pollen DNA metabarcoding to identify the floral composition of honey. How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for the...

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Is there a method to recognize all species from a mixed PCR product?

Hi everybody, I used universal primers to find the origin of plant or plants made material by insect and now I have a mixed PCR product of several plants. Is there a method to recognize all...

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Has anyone had success with an in-house von Willbrand Factor collagen binding assay (Type I)?

Currently using vWF:CBA Collagen I 96/well kit in a research lab. They are pretty expensive, and sometimes have issues of unavailability. Has anyone had success with a recipe for an in-house assay?

10 November 2017 5,104 0 View

What is a good high molecular weight marker for evaluating vWF?

I'm a little new to this, so please forgive me. In electrophoresis and Western blotting to evaluate von Willebrand factor in agarose gel, what would be a good high MW marker?

09 October 2016 4,377 3 View

Can multiple freeze-thaw cycles be performed on a hydrogel (PVA/PVP) for crosslinking without changing the mechanical properties?

The polymer goes through 6 freeze-thaw cycles (21 hours at -19C and 3 hours at 25C). Does anyone know, if I perform 2 or 3 cycles in a day, would the polymer be the same?

03 April 2013 7,457 1 View

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