How do you prevent excessive foaming of organic-matter-rich soils during H2O2 digestion?

My samples are very high in organic matter, and even small amounts of H2O2 cause the samples to foam up 4-5 times the beaker volume, spilling onto the lab bench. Is there anything that can be done...

17 January 2021 8,784 3 View

Does it matter if antibodies are monoclonal or polyclonal for Quantitative Western Blots?

Hello helpful people, I am planning a quantitative western blot experiment and have the choice between a polyclonal and monoclonal antibody. I have never done western blots personally. Does...

11 November 2020 780 4 View

How to determine fluorescence quantum yield for photoswitchable/photochromic compounds?

How to determine FQY for photoswitchable compounds? I found most related publications didnt mention, or just said that they used "Absolute quantum yield spectrometer." I think such technique...

31 October 2020 8,002 5 View

Will intron-mediated enhancement of gene expression affect downstream of a fusion protein?

Hello everyone! I'm currently trying to increase the expression of my fusion protein in a C. elegans animal model. After reading papers, I found two possible methods to try: (1) codon-optimization...

29 September 2020 490 2 View

Should we include 2 clinically correlated variables (Correlation constant = 0.8) in the same Multi-linear regression model?

I am currently studying the effect of atrophy of a muscle on the clinical outcome of joint injury. There is actually another muscle that was previously well established to have an effect on...

06 September 2020 9,986 7 View

What could be an explanation for making plants in their growth development appear fake growth rings?

As plants grow in their development, they undergo many changes either due to climate change or other environmental issues that affect plants but I want to know how false growth rings form and why...

16 December 2019 1,615 5 View

Primers for amplifying Lactadherin?

Has anyone had previous success in amplifying Lactadherin from the addgene construct below? https://www.addgene.org/46830/ I'm looking at the sequence and it seems that the starting sequence of...

11 October 2019 1,158 3 View

What is the best way to train rodents to perform complexe behavioural tasks in a virtual reality environment?

I would like to know what is the best way to train mice in a virtual environment to perform behavioural tasks (to run consistently, to train them to reach a specific target without stops before to...

09 September 2019 3,902 4 View

Is there a straight forward way to modify the amine of an amino acid?

It's been a while since I've thought a ton about synthetic organic chemistry, so please for give my naivete. For example, say I wanted to add an R group to the amine group on glycine. I believe...

24 August 2019 10,011 3 View

Why would media stain cells?

I'm working with mesenchymal stem cells, and made an observation that I'm scratching my head to explain. After spinning down cells and washing in PBS the cell pellet for WT cells is white, but...

06 January 2019 4,035 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View