I would like to add Fungin (Invivogen) to my MS medium to control fungal contamination. Unfortunately, I cannot find any documentation for the product that says whether to add it before or after autoclaving the medium. I don't imagine it is heat stable, but I wanted to double check before I go through the hassle of adding it to individual culture tubes. If it is not heat stable, how cool should the medium be before I add it? Can I add it to cooled medium immediately before culturing explants?
Thank you!
Robert Adolf Brinzer
Best to add thermolabile components like antibiotics and antifungals after autoclaving. Use a laminar flow hood to add to room temperature media just before use.
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Eric Perouzel
Fungin is heat stable up to 100 C but it will not resist autoclaving....
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