How do you prevent excessive foaming of organic-matter-rich soils during H2O2 digestion?

My samples are very high in organic matter, and even small amounts of H2O2 cause the samples to foam up 4-5 times the beaker volume, spilling onto the lab bench. Is there anything that can be done...

17 January 2021 8,784 3 View

Does it matter if antibodies are monoclonal or polyclonal for Quantitative Western Blots?

Hello helpful people, I am planning a quantitative western blot experiment and have the choice between a polyclonal and monoclonal antibody. I have never done western blots personally. Does...

11 November 2020 780 4 View

How to determine fluorescence quantum yield for photoswitchable/photochromic compounds?

How to determine FQY for photoswitchable compounds? I found most related publications didnt mention, or just said that they used "Absolute quantum yield spectrometer." I think such technique...

31 October 2020 8,002 5 View

Will intron-mediated enhancement of gene expression affect downstream of a fusion protein?

Hello everyone! I'm currently trying to increase the expression of my fusion protein in a C. elegans animal model. After reading papers, I found two possible methods to try: (1) codon-optimization...

29 September 2020 490 2 View

Should we include 2 clinically correlated variables (Correlation constant = 0.8) in the same Multi-linear regression model?

I am currently studying the effect of atrophy of a muscle on the clinical outcome of joint injury. There is actually another muscle that was previously well established to have an effect on...

06 September 2020 9,986 7 View

What could be an explanation for making plants in their growth development appear fake growth rings?

As plants grow in their development, they undergo many changes either due to climate change or other environmental issues that affect plants but I want to know how false growth rings form and why...

16 December 2019 1,615 5 View

Primers for amplifying Lactadherin?

Has anyone had previous success in amplifying Lactadherin from the addgene construct below? https://www.addgene.org/46830/ I'm looking at the sequence and it seems that the starting sequence of...

11 October 2019 1,158 3 View

Is there a straight forward way to modify the amine of an amino acid?

It's been a while since I've thought a ton about synthetic organic chemistry, so please for give my naivete. For example, say I wanted to add an R group to the amine group on glycine. I believe...

24 August 2019 10,011 3 View

Why would media stain cells?

I'm working with mesenchymal stem cells, and made an observation that I'm scratching my head to explain. After spinning down cells and washing in PBS the cell pellet for WT cells is white, but...

06 January 2019 4,035 3 View

How do you diagnose and eliminate contamination?

Some of my cell cultures are contaminated, showing black spots that seem to multiply. The cells round up, and some of them appear to have granular membranes. Any ideas what is causing this and...

22 July 2018 6,839 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View