I am trying to measure cellular ROS using CellROX Green while also immunophenotyping and looking at some surface activation markers (CD69, CD86, CD138). Wondering if anyone has tips on fluorophores that worked well or if I should plan to do multiple experiments (one to see if ROS is different and one to look at the expression of certain cell surface markers) in order to get cleaner results. Would love any guidance if people have done similar experiments!