Hi,

I have been doing smFRET experiments for sometime now and this is the first time I faced this situation. I was using GODCAT system in my buffer during smFRET experiments as an O2 scavenging system. Now I wanted to shift to PCA/PCD.

I had no problem in using GODCAT anytime, however, when I used PCA/PCD the background just went up. And it was a lot. Can you all share your experiences and also give a probable solution?

What came to my mind is maybe the stock solutions of PCA/PCD have gone bad. They were made few months back for sure. Our lab has used PCA/PCD before for similar experiments, the only difference being that this time compared to all other previous studies, I have salmon DNA in my buffer in very low amount.

Thanks,

Kushal