I've read something about unefficient inhibition of proteasome by MG132 in A431 cell but I can't remember the manuscript. Could you help me?
I did this experiment with MG132 (15 uM) in A431 cells. Please check my paper:
Article Efficient Blockade of Akt signaling is a determinant factor ...
Given that both quantum yields and external quantum efficiencies are defined by the ratio of emitted to absorbed photons, experimentally, what is measured using the relative and absolute...
25 April 2024 934 1 View
Good morning, I uploaded a wrong publication: how could I correct the error, that is modfy the articles uploaded e modify them? Thank you Yours sincerely Domenico Viola
24 September 2023 9,739 1 View
I am developing a scale for volunteer motivation with 10 dimensions, where 6 dimensions with low Cronbach's Alpha (0.507, 0.651, 0.681, 0.663, 0.621, 0.654). The other 4 dimensions' are okay...
01 June 2023 9,822 2 View
I am confused about removing items in EFA and couldn't find any guidance paper. I tried to delete one item every time. Therefore, I am able to delete the ones I don't want (different from what I...
25 May 2023 1,739 9 View
Some of the factors are correlated and some are not, then how can I choose the rotation methods? I have tried both. With oblique rotation, too many factors were generated. Orthogonal rotation...
23 May 2023 1,308 3 View
More specifically, starting on August 2022, EasyChair only allows small conferences (max 20 submissions) for free. Would you reccomend other similar services?
17 October 2022 2,343 1 View
Not really much to add to the question itself. It is quite established that neurotypical humans are particularly good at DETECTING* faces. Of course there are false positives, i.e. we sometimes...
03 April 2022 4,163 3 View
I am looking for an ELISA kit working on sheep for oPL detection in serum or plasma samples. I suppose human placental lactogen kits could be a choice. I didn't find anything in literature...
28 October 2021 1,613 3 View
I'm trying to find the most simple conditions to quantify nicotine in e-cigarette liquid samples. I have an HPLC-UV (Shimadzu LC2030C plus) with a C18 column (VP-ODS shim pack, size 150l x 4.6, ID...
06 July 2021 2,163 2 View
Hello, when i run a solvent solution there is a peak at the same time where my fragment ions of compound A elute. how i can eliminate this peak from solvent solution? This peak is not from carry...
22 June 2020 335 1 View
In 2024, which one is the most significant target for CRC drug discovery?
01 June 2024 703 2 View
Collaboration and joint research Call: Recently we conducted studies on BRCA1, BRCA2, EGFR, and ESR1 mutations in cancer! Our computational approach identified potential pathogenic variations...
19 May 2024 2,012 3 View
I cultivate CAF from the specimen of the pancreatic cancer patient who had pancreaticoduodenectomy (PD). Other than the medium, FBS, antibiotics, is EGFR needed for the culture?
07 April 2024 8,937 0 View
Hello, I am wondering if any of you experts could help me with my peptide design. I want to design a peptide that can penetrate the skin cell and specifically target the epidermal growth factor...
01 April 2024 3,178 1 View
Dear All, I use HEK293-T cells for preperation. I treat them 24h with epoxomicin. And then I isolate proteins using RIPA buffer. I precipitate overnight 5 mg protein with 25 micro liter of...
29 March 2024 3,436 0 View
Hello¸ Im PhD Student in UQTR ( Canada), I have a crucial experience for my project thesis : I have a WB (western blot) membrane that I need to store for a long time for incubation with other...
25 February 2024 7,082 1 View
Hello, I'm curious about the in vitro experimental method used to assess whether or not some kinase enzymes directly phosphorylate other kinase receptors, such as EGFR. Which experimental method...
19 December 2023 9,428 2 View
I want to test the efficacy of a custom made EGFR antibody via ELISA. Can anyone suggest me the best way to do it. Thanks Nidhi
03 December 2023 9,003 2 View
In our in-vivo assays, we see a modified band which we know is a mono-ubiquitin band. We still want to confirm with a mono-ubiquitin specific antibody, if the same is available commercially.
19 November 2023 7,374 0 View
In recent years, various strategies for targeted protein degradation have been developed using the ubiquitin-proteasome system or the autophagy-lysosome system (PROTAC, LYTAC, etc.). However,...
22 September 2023 6,585 0 View