How to eliminate a peak in solvent which elutes at the same time with a compound in standard solution using LC-MS/MS?

Hello, when i run a solvent solution there is a peak at the same time where my fragment ions of compound A elute. how i can eliminate this peak from solvent solution? This peak is not from carry...

22 June 2020 172 1 View

International publishers in categories A, B, or C?

I need to know what are international publishers in categories A, B, or C according to the classification of the Research School for Socio-Economic and Natural Sciences of the Environment...

13 May 2020 476 28 View

Where does the notion of "Central Nervous System" (including both the brain and the spinal cord) comes from?

I mean, you can easily find out that Galen was the pioneer, correcting Aristotle; and a bunch of other intersting historical things. But WHO exactly decided to establish this partition, WHEN, and...

11 March 2020 5,083 2 View

How does a wrong (higher) concentration of loading buffer affect the results of a gel electrophoresis?

In case that the loading buffer (6x) was not diluted to 1x. It was a 2% agarose gel.

03 March 2020 1,406 4 View

What if resting state networks only tell us a neurovascular (rather than neural) story?

If I've understood it correctly, a recent paper by Aaron Winder and colleagues (Weak correlations between hemodynamic signals and ongoing ne... ) shows that in mice altered cerebral blood volume...

03 December 2017 5,264 4 View

Which is the best protocol to extract RNA from tomato fruits?

I am working with a big number of ripe fruits so a fast method is a plus. I am also wondering if it is better to freeze dry the samples to preserve their quality.

27 September 2017 8,670 3 View

I am trying to establish stable cell line with HepG2 cells.Does anybody have experience with that?

I have been trying to establish a stable cell line using KnockoutTM Single Vector Inducible RNAi System from Clontech. I used Lipofectamine2000 for transfection and then I have selected the cells...

15 September 2015 238 2 View

Can somebody explain what Deleuze and Guattari's adoption of Artaud's 'Body Without Organs' means in terms of its relationship to Schizophrenia?

I am interested in the concept with regards to Capitalism, Neuroscience and Hallucinogens.

27 December 2012 2,189 3 View

Application of computer vision system in food product colour measurement

Is there any research update on the CIELAB colour measurement of food products using any latest colour analysis software? let us have a discussion on this.

01 January 1970 5,439 1 View

How can one achieve a free flow powder when using a raw material that has a high rate of agglomeration after drying and milling?

The raw material is a root vegetable.

01 January 1970 5,872 3 View

Why do I see variability in mRNA expression in qPCR of the same gene (ubiquitin)?

Hi everyone! I use HaCaT cells and i transfect them with siRNA. After 48 h I extract proteins with Laemmli and RNA with trizol. I proceed with western blot to check proteins levels (and they are...

26 January 2021 5,738 3 View

Hiv1 integrase western blot detection?

09 October 2019 4,016 3 View

Can anyone recommend any articles with clear ubiquitin staining in cells?

My ubiquitin staining is fairly variable and I've been looking for published articles containing such stainings so I can see how it normally looks. I've had some trouble locating any good sources...

14 September 2019 8,904 1 View

Can anyone recommend any articles with clear ubiquitin staining in cells?

My ubiquitin staining is fairly variable and I've been looking for published articles containing such stainings so I can see how it normally looks. I've had some trouble locating any good sources...

08 September 2019 9,393 1 View

Could anybody recommend me an ubiquitin antibody?

Hello, we are trying to detect changes in ubiquitinylation on our protein of interest. Has anybody any experience with ubiquitin antibodies and could recommend me one? We are preferably...

02 March 2019 2,636 3 View

Do you know if MDA-MB-468 (EGFR-) cell line exists ?

Hi I am working on MDA-MB-468 EGFR(+) and i want to find the same cell line but without EGFR (-) ? I did some research on NCBI but i didn't find an article when someone use it. If anyone have...

02 March 2019 9,345 3 View

What is the most dependable (reliable) factor to determine the tumour microenviroment difference between different types of cancer?

The tumour microenviroment is very complex and composed from many bio-factors such as (EGFR, VEGF, chemokines, leuckocytes infilitrations...etc). I did many investigations on couple of tumour...

11 December 2018 452 0 View

Anybody know how long after removing Arabidopsis seedlings from a MG132 treatment I have to wait to have a fully active proteasome?

I wanna do a CHX chase assay but before the CHX treatment I wanna pre-treat the seedlings with MG132 to prevent degradation before the assay start.

11 December 2018 1,039 0 View

Do you have any experience with HS578BST cell line?

Hi everyone, I am trying to do some experiments with HS578BST cell line (normal breast cell line) but I have big problems on growing them. I am using DMEM (with 10% FBS) and EGFR supplied every...

16 November 2018 9,218 3 View

Do you have any experience with HS578BST cell line?

Hi everyone, I am trying to do some experiments with HS578BST cell line (normal breast cell line) but I have big problems on growing them. I am using DMEM (with 10% FBS) and EGFR supplied every...

10 November 2018 9,831 4 View