Has anyone examined the ferret pup retinal vasculature?

More Ellen Ingolfsland's questions See All

What is the best ratio for practical skills and theory for full time students?

When teaching vocational subjects we have to balance between theory and practical skills for students to progress to next level.

19 May 2020 4,367 3 View

Minimum levels of COVID detection?

Hello good people, This is not a normal question for me, but a friend in Public Health wanted to know, so I thought I would ask. The test for COVID is a PCR, if I am not mistaken. The question...

16 March 2020 3,322 2 View

How to Find Demographic Data on Social Media?

I'm a research assistant on a project tracking teen social media usage. We need to find statistics on age demographics for various social media platforms (Twitter, Tik Tok, etc.), but so far,...

15 September 2019 5,881 2 View

How to clone a gene with long sequence?

Dear All, I'm now trying to amplify a gene with 3690bp from the genomic DNA. I've design 3 sets of primers. First set is flanking the whole gene. Second set is flanking from 1 to 2613bp. Final...

07 February 2019 1,167 1 View

How do I remove something that publisher complained about?

I cannot share copyright book. Mary Ellen Gioldberg

19 January 2019 7,782 3 View

Looking for ways to include water quality microbiological parameters into a statistical analysis using censored data analysis methods?

Interested in finding out other peoples experiences in dealing with microbiological parameters (such as Faecal coliforms, total coliforms, etc) are are below the method detection limit of the...

12 January 2018 7,546 2 View

Anybody have resources (article) for predicting via logistic regression modelling for an environmental contaminant above a threshold concentration?

Environmental Logistic regression modelling Contaminant Machine learning

29 December 2017 1,741 4 View

Good protocol for DNA/RNA Immunoprecipitation (DRIP) detecting R-loops?

I'm trying to do a DNA/RNA immunoprecipitation (DRIP) using the S9.6 antibody and am getting no DNA pull down. I isolate DNA from cultured cells using the Qiagen DNeasy Blood & Tissue Kit and...

07 September 2017 148 3 View

What do you think of rapid curriculum change?

In the philippines, the 10-year basic education has changed to Kto12 curriculum. i need to know the issues behind rapid curriculum change and the things needed to prepare for such change.

16 February 2017 9,169 12 View

Does anyone have experience or advice on analyzing a population?

We have 8 years of data that includes info on all crimes reported to MPs and all crimes the commanders submitted for prosecution. This is not a sample - it is the entire population of crimes...

19 May 2016 347 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View