Has anyone examined the ferret pup retinal vasculature?

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What are the inclusion and exclusion criteria for selecting studies in the meta-analysis?

According to the study of Pan et al. (2023) entitled "Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis," what are the...

08 April 2024 3,776 1 View

Whats is the best protocol for spheroid dissociation?

I'm doing primary tumor spheroids to a drug screening. We're having success to make the spheroids but I'm not having succes to dissociate the spheroids to evaluate the cell viability (using trypan...

18 July 2023 2,015 1 View

How to format qPCR data for geNorm?

Hi there, Has anyone successfully used GeNorm, one of the free programs to determine best reference gene(s) for qPCR? I cannot figure out how to format my qPCR data to get the qbase+ program to...

28 February 2023 7,677 2 View

Why won't EDC dissolve in Poly(acrylic acid)?

I'm trying to thiolate Poly(Acrylic acid) (PAA) with L-cysteine using EDC (or EDAC), however prior to the addition of L-cys, when I add EDC to PAA large clumps form in solution. 1g of PAA was...

01 February 2023 6,701 2 View

Refractive Index for Dako fluorescent mounting media?

Agilent Cat S302380-2, Dako Fluorescent Mounting Media I've checked the Agilent site and found no details regarding the product (and their chat bot is down), and specifically, the refractive...

26 January 2023 7,422 0 View

Methods to measure vascular leak?

I am trying to decide between different methods to compare vascular leak in the retina between different rodent groups. I have found a number of different methods, inlcuding immunofluoresence for...

25 October 2022 9,457 0 View

Target amplitude cannot be reached?

I work with a JPK Nanowizard 4, and though the calibration spectrum looks fine, and the corresponding resonance frequency reasonable, when I run AC Feedback Mode Wizard to select the resonance...

30 August 2022 941 0 View

How to analyse a shannon diversity index of two conditions and 2 years?

i am trying to analyse the diversity of sites in two conditions (protected area v non protected) and from 2021 to 2022. i have worked out the shannon diversity of each site but unsure on how to...

19 July 2022 3,284 0 View

What test can i do with shannon diversity indices of two unequal samples?

i have two condition and looking at comparing their diversity. however, i have 13 sites in one condition and 9 in the other. i calculated the shannon diversity index for both conditions, but as...

17 July 2022 4,585 0 View

What to do with heteroscedasticity for a linear regression analysis?

I seem to have a hard time with the statistics for linear regression. I have been scrolling on the Internet, but did not find an answer. I am testing the assumptions for linear regression, of...

10 March 2022 1,324 8 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

What can be the minimum number of animals (rats) to be used for a pilot/preliminary study on intradermal drug delivery?

Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?

02 August 2024 4,570 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View