Has anyone ever worked with the SW48 cancer cell line? Any tips on its culture?

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Zuzana Jaskova

Hi,

I often work with SW48 cell line and there is no need for special handlings with this culture. We culture them in 5% CO2, 37´C in DMEM supplemented with 10% FBS, penicilin-streptomycin, glutamine, and sodium pyruvate . Also, no trypsin is required, they are OK if a cell scraper is used.

Good luck with experiments!

Eric Smith

The CO2-free environment would be necessary if you grow them in Leibovitz's L-15 Medium. The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation.