I would like to work with this cancer cell line, but I found odd culturing requirements, such as working in a CO2-free environment, whereas in different publication they have been cultured in standard conditions.
Hi,
I often work with SW48 cell line and there is no need for special handlings with this culture. We culture them in 5% CO2, 37´C in DMEM supplemented with 10% FBS, penicilin-streptomycin, glutamine, and sodium pyruvate . Also, no trypsin is required, they are OK if a cell scraper is used.
Good luck with experiments!
Hi Jacopo, ask to Fulvio Chiacchiera.. he has a great experience with them ;) Andrea
Thanks Zuzana and Thanks Andrea for your quick a thorough answers! :)
Get in touch with Jose Solano . He could help you. He has been working with this cell line.
The CO2-free environment would be necessary if you grow them in Leibovitz's L-15 Medium. The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation.
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