Has anyone ever successfully transfected induced pluripotent stem cells (iPSC) using Gene Pulser electroporator from BioRad?

21 January 2022 1 1K Report

I do not have a nucleofactor available and I am trying to get it with Gene Pulser. But maybe I am having issues to get the right setting on the equipment or it just really wont work... Was anyone able to transfect PSCs using this equipment?

Ignacio Rodriguez Polo

Dear Sheron Cogo,

Yes, it is possible to transfect them. However, I usually get lower efficiencies than with the nucleofector.

I use a single 20 ms square wave pulse (250 V).

There are several publications using different protocols that you can try, eg,.:

https://www.nature.com/articles/s41467-020-15042-0

https://www.cell.com/stem-cell-reports/pdf/S2213-6711(17)30552-0.pdf

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5905711/

Good luck!. Best,

Ignacio

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