DMEM - dulbecco's modified eagle's medium, consisting of the typical Amino Acids, Glucose, pH indicator, Salts and Vitamins
We want to find a technique for determining of glucose concentrations in this culture from impedance spectroscopy data
Hi look for this paper......Biomed Mater. 2009 Dec;4(6):065011. doi: 10.1088/1748-6041/4/6/065011.
Influence of artificial biological fluid composition on the biocorrosion of potential orthopedic Mg-Ca, AZ31, AZ91 alloys.
Hi Mikhail
We measuared biological object (fruits) during their putrefaction process on three frequencies 20, 100 and 500 kHz . Typical patert what we have achive after interpolation provided in:
researchgate.net/publication/261914659
Dear Michail,
the only thing I and my students have ever measured in the filed of biology was South African frog epithelia. There, EIS was very useful. So, in principle, I would say yes, Impedance Spectroscopy is the fullest and most sensitive electrical characterisation tool there is (both in semiconductor physics, electrochemistry and biology and medicin). Whether you can extract glucose concentration from the EIS data I do not know.
My only general comment would be, regard EIS just as you would regard one of the optical spectroscopic method (IR.VIS,UV,..). That is never measure EIS at one, two or few frequencies, measure the electrical impedance in the entire frequency range . It is there, the most complete and full information lies. It might be that Vitaliy got some useful information, measuring just three frequencies, but that information is very incomplete and subject (almost certainly) to appreciable errors during interpretation.
With best regards
Petr
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