What would my sampling technique be?

I am having issues explaining my sampling technique in my methodology. I have utilised a two phase sequential explanatory design collecting and analysing the Quantitative data in the form of an...

01 August 2019 4,049 4 View

MG-63 plasmid transfection using Lipofectamine 2000?

Has anyone got any experience of transfecting plasmids into MG-63 using Lipofectamine 2000? I am currently using it to transfect three GFP-containing plasmids - one of which is 7kb and the other...

06 February 2019 3,663 2 View

What may cause the death of mice after repeated immunization with OVA + alum?

I have tried to induce an allergic response in female Balb/c mice. Mice were immunized i.p. with OVA + alum 3 times (days 0, 10, and 20), and after the 3rd immunization, mice started to die. Does...

04 October 2018 5,171 4 View

Using regepochs function to slice the eeg data collected from Emotiv Epoc+ in EEGlab?

Hi all I am completely new to Matlab and EEG, and I've encountered an issue while I was trying to slice a continuous data with no marker captured using Emotiv Epoc+. I am using Matlab R2017b...

20 March 2018 3,075 1 View

Light wavelength attracting pest natural enemies?

Now I am interesting in if any kind of light wavelength could usefully attract pests natural enemies like lady beetle, predatory bugs, hover fly, lacewing, or parasitoids. Could any one could...

10 November 2017 894 3 View

1000 fold increase compare to control in real time PCR, is that normal?

When I over expression a protein and detect the increase of mRNA of that protein using real time PCR, the result show increase 1000+ fold to mock transfection sample. Is this result common and...

26 December 2016 6,923 5 View

If one variable (moderating) is positively skewed, how do you conduct parametric testing?

* Efforts to transform the variable via log and sqrt were unsuccessful

21 May 2016 761 3 View

Should the moderating variable be correlated with the independent and dependent variable?

21 April 2016 2,994 7 View

What can be the problem for very low or non-detectable expression after lentivirus infection?

16 August 2015 5,980 3 View

Is Thrips dangerous for Prunus Mume?

16 April 2015 3,718 2 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View