Arun Pandian Chandrasekaran

7 Questions 17 Answers 0 Followers

Questions related from Arun Pandian Chandrasekaran

Has anyone attempted to transfect ES cells with Cas9 RNP/sgRNA/ssDNA using Lipofectamine?

I am attempting to insert a reporter gene into human embryonic stem cells using Lipofectamine-based reagents such as Lipofectamine 3000, CRISPRMAX, and RNAiMAX. Has anyone had success with these...

26 March 2023 4,090 0 View

Why is Fluorescein more fluorescent than Phenolphthalein, at high pH?

If both Fluorescein and Phenolphthalein are at alkaline pH, why cant phenolphthalein fluorescent at that pH? Many thanks in advance.

06 August 2015 3,392 4 View

Can anyone suggest a review or research article on the climatic impacts on human health?

Emission of air pollutants from any means will be a threat to living things. Please suggest some statistical data on how the humans are being affected by pollutants over the decades. Any sort of...

26 February 2015 1,601 3 View

How can I calculate crystallinity index (CI) from XRD data?

I have seen some methods in web but it seems to be difficult. I am a beginner to this kind of work. Any suggestions from your side would be very helpful. Thanks in advance.

17 January 2015 9,195 12 View

Can someone help with this problem in nano composite material synthesis in order to check their anti-microbial activity?

I'm raising this question on the behalf of my colleague. She is working on nano composite material synthesis.She wanted to check anti-microbial activity of the composite. Whenever, she...

13 January 2015 5,040 4 View

Do the same enzymes from different sources affect the reaction in any manner? Can someone explain it elaborately?

For an example, Will there be any difference in terms of product conversion if I use Cellulase from different sources like Trichoderma reesei, Aspergillus niger, etc. ? I am working on enzymatic...

20 December 2014 1,506 9 View

We know that algae are capable of producing large amounts of biomass and oil that can be convert to bioethanol or biodiesel but at what percentage?

Is there any specific strain of algae available for producing bioethanol or biodiesel alone? What is the percentage composition of biomass and/or lipids in a typical alga? Please clarify this.

26 November 2014 4,795 10 View