Tara Mchugh

4 Questions 4 Answers 0 Followers

Questions related from Tara Mchugh

Has anyone attempted adding metals (lead nitrate, etc) to tissue already mounted on Synaptek grids for EM?

TEM imaging

31 May 2024 3,843 2 View

Does anyone use PEG + ethanol to store brain tissue at 4 degrees C?

Trying a new way to store human brains. I do not have experience with PEG. Any idea how the tissue appears next to tissue stored in anti-freeze? Thanks

10 April 2023 4,115 0 View

Has anyone had problems keeping lead aspartate staining solution in solution?

We're following a few different protocols based on Deerinck's work making a step in electron microscopy preparation with lead nitrate and aspartic acid. My question is what is more important,...

17 May 2017 1,028 3 View

Is it possible to refix a human brain after cryostat protecting it?

I am not familiar with the fixation process that the brains have been subjected to you. However, we have placed them in -80 C to ready for sectioning, but the tissue falls apart. Any suggestions?

14 October 2015 2,254 5 View