Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View

How do I correct an inaccurate citation?

I was asked to verify a citation: Drugs and galencials, by D. C. Garratt, B.Sc., Ph.D (Lond.),... The author's first name is Emerson and the last name is Beeler. It was incorrectly cited as...

03 February 2021 5,272 3 View

Is there a way to get rid of TEA in a TLC plate in order to do proper staining?

Dear colleagues: I have been trying to establish a few HP-TLC methods in the lab. In general, for quantification purposes, I prefer CuSO4+acid charring, to primuline staining. Lately I have been...

20 January 2021 9,019 1 View

I have a challenge with a structure I optimized it's weight but the frequency constraint is not satisfied?

I know that frequency depends on material properties, geometry, support conditions and in plane loading. I have played around with these figures except that I maintained the same material steel...

26 December 2020 4,877 9 View

How can I coat reduced graphene oxide on glass substrate?

I dispersed reduced graphene oxide in NMP solution and tried to coat it on a glass substrate using dip coating system. But I am not able to get a uniform coating. Please suggest me a solvent and a...

26 November 2020 8,929 4 View

How can I measure the sheet resistivity of my reduced graphene oxide thin film?

I tried to coat my NMP dispersed rGO sample on the glass substrate by dip coating system. Then I characterized it with four probe system for sheet resistivity measurement. But I got a very high...

16 October 2020 3,696 3 View

Heat and plastic in MTBE lipid extraction?

When extracting lipids with the MTBE method, the indicated temperature is RT. In addition, drying the samples in a speedvac is mandatory due to the presence of water and such procedure is long and...

14 October 2020 9,776 2 View

Does anybody have an idea of how to solubilize hydrophobic peptides?

My hydrophobic peptide (12 AA) is soluble in 100% DMSO. Given that I should encapsulate it in hyaluronic acid nanoparticles, I need that peptide is soluble or partly soluble in water. Does someone...

03 March 2020 8,025 4 View

How do I choose which EEG trials to run in Common Space Pattern (CSP)?

I am currently working on a binary classification of EEG recordings and I came across with CSP. As far as I understand, CSP allows you to choose the best features by maximizing the variance...

20 January 2020 7,536 2 View

For researchers who have a P-97 Micro-pipette puller from Sutter instruments, can you help me get the values of factory pre-programmed Program #1?

We have lost the parameters of this program by mistake in our lab. And we need to reenter those, to our new P-97 puller. A picture would be good. I appreciate the help.

15 October 2019 5,710 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View