Has anyone already used a Qiagen GeneReader and can comment on it for NGS?

More Rita Spilka's questions See All

Differentiation of KOLF-NGN2 hiPSC into neurons - Troubleshooting?

Hello everyone, We are trying to differentiate the KOLF-NGN2 hiPSC into neurons - A reference human induced pluripotent stem cell line for lar... . The differentiation is characterized by 2...

09 July 2024 4,002 1 View

Hello. Is it possible to perform a moderated path analysis on Amos when the selfish variable is multicollinear?

Moderation, multicollinearity

30 April 2024 9,574 5 View

Factorial Survey Method: Will two vignettes be sufficient for a 4 x 2 study with a sample size of 385 survey participants?

Searching for articles or insight on determining the number of vignettes needed for a study using the Factorial Survey Method.

04 March 2024 1,680 3 View

Which mouse can be used better for the study of respiratory sensitization/asthma: BALB/c or BALB/cByJ?

We would like to order a BALB/c strain from Charles Rivers or Jackson laboratories, but apparently there are two such strains, and we are not sure which one is better or if there is any difference...

09 November 2023 5,876 0 View

Arbitrating and Mediating Generative AI Works: The Legal and Ethical Challenges?

Arbitrating and Mediating Generative AI Works: The Legal and Ethical Challenges The jurisdiction doesnt really matter i am looking at the possibility of decongesting the docket of the court and...

12 August 2023 6,835 5 View

How can I create a free DOI?

05 August 2023 6,507 1 View

What is the composition of RNA prep buffer in RNA isolation kit by Zymo?

RNA isolation

29 June 2023 5,130 2 View

Can I get an assistance from the researchers working in EMI shielding to check my Code used to measure complex permittivity using NRW method?

I used MATLAB code on NRW method to measure the complex permittivity of my nanocomposite samples. The results I got using it were very unexpected. I rechecked my code many times but did not...

15 May 2023 3,955 0 View

The best method to detect EGFP in mouse skeletal muscle after dissection?

We have infected mice with an AAV-EGFP and we were hoping to directly visualize the fluorescent signal in frozen muscle sections without antibody staining. Any suggestions would be greatly...

12 April 2023 4,058 3 View

What are some of the Strategic Management Accounting Practices in Developing Countries??

A sample of this Question need to be answered in a literature review form

27 February 2023 5,613 11 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What should a Mechanical Engineering PhD scholar focus on during their PhD to enhance their chances of securing a postdoctoral position?

29 July 2024 7,714 4 View

May I know the exact Quartile of the journal- Advanced Engineering Materials (Wiley) for material science category?

In some data sources it has been grouped in Q1 and some shows it is Q2.

29 July 2024 4,227 2 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View