Hello everyone, i am looking to use the RPTEC cells to develop the stable knockout/knockdown cell lines. But as i am going through culture maintenance protocol for RPTEC cells, i was observed its difficult and costly to maintain them and also they differentiate if they are in culture for 15-20days. I thought this will affect them during the selection process while generating stable cell lines. Thus i am wondering if anyone has already developed stable knockout/knockdown cell lines from RPTEC cells?

thank you