We use athymic nu/nu mice for our SKOV-3 xenografts (http://altogenlabs.com/xenograft-models/ovarian-cancer-xenograft/sk-ov-3-xenograft-model/). Using matrigel is a plus, and our initial dosage and general procedures are published on our website. Usually the tumors take a few days to grow to a sufficient size to begin drug dosages, and another few weeks (just over a month) for the study to complete (based on the tumor size).

SKOV-3 Ovarian tumour xenograft have proven challenging to grow. I have tried several times without sucess. I know of collegues at other institution who have sucessfully used SKOV-3 xenografts but both reported to me that the initial take rate was very low and, in order to acheive a usable number of tumour bearers, the initial tumours were passaged as lumps in nude mice which can be very expensive.

The initial cell number was 10 to 6 cells per mouse which took upto 6 months to produce a 1 cc tumour. Subsequently a 1 cc tumour was passaged in to 3-6 mice to maintain the xenograft line.

Hope that helps

Thanks Huw Thomas..can u please tell me about strain of NUDE, they have used(Nu/Nu or CD1 or any other NUDE mice?).

Even i am not clear with your answer about Cell no./mouse..is it 10^6 cells per mouse?

Please reply...thanks

We routinely use CD1 Nude (nu/nu) I think one group also use CD1 nudes the other used Balb-c nude which are much more expensive.

hello Milind Sagar

I can not use the estrodial pellet, because of the price, so I have a plan to make MCF-7 xenograft model w/o estrodial pellet

Hello Vahid,

I have replied to your query in peter's question link.Please check out..hope that will help you..

Thanks,

milind

Thanks a lot

Hello, by any chance does anyone know if SKOV-3 cell lines can produce ascites when injected to nu/nu mice?

It's really hard to grow tumors by SKOV3. I used 5E6 cells in the mixture of PBS and matrigel(1:1) then it took near 2 months to grow to the size around 100mm3. By the way, the mice model I used is NMRI-Foxn1nu /Foxn1nu

We use athymic nu/nu mice for our SKOV-3 xenografts (http://altogenlabs.com/xenograft-models/ovarian-cancer-xenograft/sk-ov-3-xenograft-model/). Using matrigel is a plus, and our initial dosage and general procedures are published on our website. Usually the tumors take a few days to grow to a sufficient size to begin drug dosages, and another few weeks (just over a month) for the study to complete (based on the tumor size).