How reliable is Image J for quantification of protein and analysis of Gel image (jpeg,tiff)? Has anyone used this software ever? Please kindly suggest any software used for quantification of western blot and protein Gel image.
Althoug using image J for quantifying proteins is possible, it is not a relible method for quantifying proteins after Western Blot but if you are looking to compare your target protein expression level compared with control, it might be accepted.
Works quite well and is free. See http://imagej.nih.gov/ij/ and for latest info (and plugins)and https://www.youtube.com/watch?v=KoqpCsha-UU for a good intro.