The anti-roll plate for a cryostat is a small rectangular piece of glass, yet it usually costs $100. My question is why? Is the glass special in some way? Would acrylic plastic not suffice?
I am an undergraduate mechanical engineering student seeking suggestions on topics related to either thermodynamics, fluid mechanics, heat transfer or renewable energy for my final year project....
24 July 2024 4,056 5 View
I have created a FE model including: 1. Bead (Green), 2.Cell (Red) 3.Components inside the Cell, beams, truss, and nucleus. Constraint: Embedmentt, all components embed in cell. Boundary...
03 March 2024 7,199 1 View
A interesting question: i want to create a egg model in Abaqus including 3 parts: eggshell(shell element), egg white(solid element) and egg yolk (solid element). They are wrap each other. However,...
31 January 2024 1,847 1 View
I've been imaging mammalian cells (RPE-1) post-transfection and seen the transfected cells (fluorescent) become static and do not divide, whereas untransfected (non-fluorescent) cells in the same...
27 September 2023 9,524 1 View
For reasons too complicated to get into, I'm looking to slice some CLARITY brain tissue at 40 microns. Typically, I would use a cryostat for slicing fixed brain tissue, but I don't know whether...
08 May 2023 4,001 1 View
I'm wanting to express 2 proteins (A,B) into a vector and transform cells with this. In the distributed construct strategy, each insert is cloned into a different, compatible vectors that can be...
11 March 2023 1,437 2 View
Hello, may I ask if I can gain some information to help me with my reflective practice assessment? I am in my final semester of a master's degree in mental health nursing. I am utilising the What?...
23 February 2023 1,987 0 View
These cells were split on Friday and coming in yesterday these clusters of dead cells were apparent throughout all the flasks that were split. I believe they are dead cells because some were...
12 December 2022 2,572 7 View
Hello, I am attempting to use the Adonis function (part of vegan package in R) to determine the significance of geographic location (Site) vs host species (Species) in determining Parasite...
16 January 2022 5,406 2 View
Dear all, I am want to generate a k.o. with CRISPR/CAS9 in H292 cells and have issues with the transfections. It does not matte if its for plasmid or RNP format. Does anyone have a working...
02 September 2021 1,356 2 View
I need a very narrow glass capillary for my research. How can I make a glass capillary with an inner diameter of around 200 to 500 nanometers?
08 August 2024 8,914 0 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,282 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,104 4 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 452 3 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,227 4 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,402 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,249 2 View
Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...
01 August 2024 6,133 2 View
Hello, dear colleagues, I would like your help in finding a reference that provides an average water savings due to plastic recycling compared to the consumption of virgin materials, preferably...
29 July 2024 4,630 0 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,142 2 View