Has anybody got satisfactory result from rapid amplification of cDNA ends via Invitrogen method?

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Paul J Seear

The more total RNA you use, the better the result. So ideally use 5ug, but I've used 3ug to good effect before.

Komeil Razmi

Thanks for the feedback. 3 ug seems to be a lot. So you reckon 1 ug would be insufficient?

Cheers

Although I've only had success with 3ug or more, 1ug might be okay, so give it a go if you don't have access to any more material.