Komeil Razmi

4 Questions 4 Answers 0 Followers

Questions related from Komeil Razmi

What are the common anti-DIG fluorscent for multiplex labeling?

Hello all I am currently preparing reagents for WMISH for a fish species and wondering what commercial products (Ab) are usually used to fluorscent label DIG-UTP? And if I want to label...

06 June 2019 10,064 1 View

What is the most efficient ISH protocol to labelling sperm mRNA?

Hello guys I intend to label mRNA sperm with ISH technique but couldn't find a relevant protocol. What do you think if I use ISH protocol used for cultured cells? I appreciate your feedback, in...

06 June 2019 3,434 2 View

Has anybody got satisfactory result from rapid amplification of cDNA ends via Invitrogen method?

Hello Do I need to have a 1-5 ug of total RNA to get good result from RACE by Invitrogen method?

10 October 2017 9,327 3 View

Could we extract PGC from embryonic cell line or a fresh fish embryo?

I want to have PGC line from Gambusia

07 July 2017 5,021 0 View