Hi everyone,
I really confused because I grew my bacteria cells 48 H in nutrient broth and then measured the OD 600 in a micro-plate reader. Following, I added different concentration of H2O2 (0.5, 1,5 10 mM) to each well and incubate them for 24 hours. For control I just used the 48 h old NB media containing individual strain. To day when I checked the OD 600, the absorbance was higher than the control and the 48 h old stock media.
How it comes? Can an expert explain it?
Many thanks
Dear Carole,
Many thanks for your reply. I did all experinent in replicate under aseptic condition. Maybe these papers help. Alternatively, bacteria and fungi may activate their OxyR genes and produce catalase and peroxidec enzyme to resist and survive the harsh H2O2 environment.
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