Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Despite single-cell RNA, how can I characterize a new cell subtype/phenotype?

A new cell subtype was found out only during regeneration phases after a cuprizone-induced demyelination model. How can I characterize this cell? It is important to highlight that was already...

13 February 2021 1,245 1 View

Which are the factors that determine in a tourist the destination for their travel?

I'm working on a study of how selection criteria of Tourism have changed considering Pandemic + Vaccination expectation, so propose a new prediction behavioral dynamic model for Riviera Maya (Mexico).

09 February 2021 314 7 View

Permanent Fluid temperature initialization in Ansys Fluent?

Dear researchers, I am coupling Design Optimization with ANSYS Fluent in Workbench 2020 R2. I can do the analysis. I am patching the initial Temperature values of the zones. However, the patch...

28 January 2021 4,265 2 View

Equation to calculate Chlorophylls and carotenoids concentrations from a MeOH:HCl 1% (v/v) plant extract solution?

Hello everybody! I have performed an acidic MeOH:HCl 1% (v/v) plant extraction from Arabidopsis leaves to calculate Anthocyanins concentrations based on: Rabino, I., and Mancinelli, A. L. 1986....

25 January 2021 3,686 2 View

Cronbach's alpha or McDonald's omega? Which would be more correct and in what cases?

Cronbach's alpha or McDonald's omega? When it comes to reliability, we find different methods for its estimation. Immediately, the name of Cronbach's Alpha coefficient arises, as it is the most...

21 January 2021 330 4 View

Luciferase assay for miRNA-gene: How to obtain DNA (PCR product) from the mutated primer?

To perform the Luciferase assay for microRNA-gene interaction its necessary a primer (wild-type) to amplificate the specific ligation site and also a mutated primer. But if your primer has a...

19 January 2021 8,568 3 View

Student Perception Of Cyberbullying In Social Media?

11 January 2021 8,945 4 View

Any suggestion on literature in Cyber Crime against children?

dear all I am looking for on literature in Cyber Crime against children.

11 January 2021 5,099 5 View

How to couple Moldex 3D with MATLAB for design optimization of an injection mould?

Hello, I would like to use the software Moldex 3D for the design optimization of an injection mould. How can I couple Moldex 3D with a MATLAB code? Any help appreciated, I wish you all a happy...

09 January 2021 569 2 View

Is there a recitation score bank system for fair evaluation and assessment of student recitation?

In actual and virtual class, even if there is rubric for recitation, students may still fail to receive fair grades or scores. Others are active in recitation and others are not or others may fail...

26 February 2021 8,387 3 View

Who are the possible experts I can reach out to regarding the reseach topic: Activated Carbon as Water Filter?

Hi! Me and my team mates are currently new in doing a research study. We do not have any previous experiences in research. And we just wanted to know what type of experts (the field they in) can...

19 February 2021 9,596 4 View

How to get the heading of real-time credit card dataset ?

I am working with the Credit card dataset. I am aware of the fact that the Banks and financial institutions do not share the dataset with third-party companies or researchers. I just need to know...

18 February 2021 9,186 3 View

Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View

What is required for banking institutions to shift towards contemporary marketing practices in the light of Covid-19 pandemic?

Even after the coronavirus passes, digital solutions for banks will have an enduring relevance, as customer behaviours are expected to change coming out of this crisis toward the best, while banks...

12 February 2021 4,948 10 View

How to use "filter banks" in Machine Learning to extract custom Feature Maps ?

How can I create "filter banks" for Difference of Gaussian or Laplacian of Gaussian and implement them in machine learning to exctract custom feature maps for edge/line detection ?

07 January 2021 655 1 View

How to collect data systematically?

Hello everyone, I have 430.000 data in excel file. i import the file into STATA. The dataset is all about banks States for 12 years. i need to collect 4-5 banks from each state for each year. is...

31 December 2020 4,306 4 View

Simultaneous equation model estimated with GMM that includes cross-section ad Fixed effect ?

Hello, I'm trying to replicate this model, but I don't understand a phrase of the authors. This is a simultaneous equation model estimated with generalized method of moments. The variables of...

21 December 2020 4,674 3 View

How to measure knowledge/competence gap in IT outsourcing?

Hi all, I am currently writing my master thesis about the knowledge/competence gap between an IT outsourcer and his client. By this I mean the difference of the IT knowledge and the impact that...

20 December 2020 9,228 7 View

What could be the impediments to the initiatives adopted by the bank towards FI in the Indian scenario?

this question from case Union Bank of India: Initiatives towards IT-enabled Financial Inclusion

09 December 2020 7,721 3 View