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Questions related from Duy Minh Pham
We use phenol-chloroform method to extract DNA from peanut leaves. The protocol is as followed: - Grind peanut leaves in 1% SDS in Tris buffer, incubate at 60 oC for 5 mins. Centrifuge. - Pour...
12 May 2025 9,224 2 View
Does anyone have experience with plant meristem culture? What kind of plant growth regulator combination is best? From what I read, there seem to be 2 methods. One using cytokinin (BAP, kinetin)...
09 June 2024 7,945 2 View
For phylogenetic analysis using SSRs, normally we don't use just one SSR, but a set of multiple SSRs to ensure accuracy. So how can we input these data into NTSYS? I know input for one SSR is as...
14 May 2024 1,910 1 View
In all academic sources, sucrose is identified as α−glucose (1-->2) β−fructose. However, I cannot find any explanation anywhere as to why the fructose monomer has to be in the β configuration....
13 March 2024 9,265 4 View
Normally, we use oxidative agents like hyperchlorite to sterilize plant tissue to introduce into micropropagation. However, we are doing a plant that has high phenolic contents, and the oxidative...
20 May 2023 7,677 5 View
We taught students of DNA extraction from leaves of rice seedlings. The first class got pretty good yield, with a lot of DNA precipitates clearly visible after adding ethanol 75%. Two weeks later,...
05 April 2023 5,773 3 View
I read the references, but there are still a few technical things I can't figure out: - Do you need to put the root in water? - Can the xylem sap flow be calculated per root volume instead of per...
22 July 2021 2,153 6 View
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
02 March 2021 9,962 3 View
I have this set of data: Treatment 1: 30.5; 34.5; 24.4 Treatment 2: 24.8; 20.8; 16.8 Treatment 3: 19.1; 21.4; 21.0 Treatment 4: 22.3; 26.1; 27.1...
26 November 2020 1,337 4 View
My colleague plans to do a hand pollination experiment on strawberry. This is going to be a quantitative experiment: we are trying to see how the quality of the pollens affects fruit set....
27 September 2020 7,153 3 View
I adopt primer designs from a study published in Nature ( Domestication selected for deceleration of the circadian clo... ), so the primers should be qualified. Yet in many samples, I got...
01 September 2020 10,004 8 View
In most grafting studies, I usually only see reports of the final survival rate (e.g. after 1 or 2 weeks 95% of scions survived). However, through personal experience with tomato, I observed that...
30 August 2020 6,457 7 View
Much have been known about the production pathway of ROS in chloroplast: singlet oxygen produces superoxide, which in turn produces hydrogen peroxide; both superoxide and hydrogen peroxide then...
14 May 2019 7,096 5 View
The usual method to make impression of stomata is with petroleum jelly (like Vaseline). However, I notice that after the process the area of the leaf covered with petroleum jelly withers quite a...
13 August 2018 1,871 4 View
Normally we design experiment with 3 replicates, each replicate has like 10 samples/treatment (so total number of samples n = 30/treatment). Then we average the results of these 10 samples to get...
13 March 2018 8,043 4 View
This is a related question to an older question of mine: https://www.researchgate.net/post/How_to_measure_free_phosphate_Pi_content_in_leaf_tissue. I am trying to measure free phosphate (Pi)...
12 June 2017 1,215 5 View
I want to do a petiole girdling experiment on tomato seedlings. However, I couldn't find any clear protocol to perform girdling on such young tissue. The phloem of tomato petioles also seems to...
07 February 2017 6,091 4 View
The case happened in Vietnam on August 8. A 11-day-old baby was stabbed through the head with a knife. The knife is 28 cm in length, 2.7 cm in width, went from the upper of the left eye to the...
11 August 2015 7,885 3 View
