Duy Minh Pham

6 Questions 15 Answers 0 Followers

Questions related from Duy Minh Pham

Good sterilizing agent for micropropagation of plant tissues with high phenolic contents?

Normally, we use oxidative agents like hyperchlorite to sterilize plant tissue to introduce into micropropagation. However, we are doing a plant that has high phenolic contents, and the oxidative...

20 May 2023 7,577 5 View

Reason for drop in DNA extraction yield from rice leaves?

We taught students of DNA extraction from leaves of rice seedlings. The first class got pretty good yield, with a lot of DNA precipitates clearly visible after adding ethanol 75%. Two weeks later,...

05 April 2023 5,703 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

02 March 2021 9,697 3 View

How to intepret data with significant ANOVA but not significant Tukey test?

I have this set of data: Treatment 1: 30.5; 34.5; 24.4 Treatment 2: 24.8; 20.8; 16.8 Treatment 3: 19.1; 21.4; 21.0 Treatment 4: 22.3; 26.1; 27.1...

26 November 2020 1,084 4 View

What can be the cause of nonspecific amplification in qPCR besides primer?

I adopt primer designs from a study published in Nature ( Domestication selected for deceleration of the circadian clo... ), so the primers should be qualified. Yet in many samples, I got...

01 September 2020 9,910 8 View

10 samples x 3 replicates, 5 samples x 6 replicates or 1 samples x 30 replicates, is there any difference?

Normally we design experiment with 3 replicates, each replicate has like 10 samples/treatment (so total number of samples n = 30/treatment). Then we average the results of these 10 samples to get...

13 March 2018 7,965 4 View