Good mouse VE-cadherin antibody?

More Krish Kizhatil's questions See All

Does anybody know of a cell type with enriched expression of CD63, CD9 and CD81?

I need an appropriate positive control for WB antibodies I am using to confirm the presence of exosomes in samples. The thing is I am having reproducibility issues, and I would like distinguish...

19 June 2024 4,210 3 View

How long do you leave endothelial cells to grow on Transwells before performing macromolecular permeability assay?

I want to test the effect on endothelial permeability of certain compounds. I am using Transwell filters (0.4 microns pore size), FITC conjugated dextran (40 kDa) and Human Aortic Endothelial...

20 December 2023 7,641 1 View

Is it normal to see floating particles that resemble unattached cells in adherent cell culture?

I am culturing Human Aortic Endothelial Cells from ThermoFisher (Cat #: C0065C). I am doing it with the media and supplements they sell for it, which are called M200 and LVES respectively. Despite...

17 October 2023 9,200 1 View

What is my best option to concentrate a protein solution obtained through RIPA lysis buffer?

I recently performed protein extraction with RIPA buffer of some cells I collected. The intended purpose was to use it to load in an SDS PAGE gel for subsequent Western Blot. I made the mistake of...

20 September 2023 7,625 4 View

Any suggestion for why I have inconsistent bands for my loading control on Western Blot?

Samples were anti-CD3 and anti-CD28 co-stimulated Jurkat cells. The gel was 10% acrylamide SDS-PAGE. Semi-wet transfer to nitrocellulose membrane using TransBlot Turbo system. Membrane was...

11 October 2022 6,917 4 View

How do I perform Hydrogen bond analysis using readHBmap.py?

Hi all, I have used the below code for analyzing hbonds in my protein, but I am facing the error below. Please provide solution for this error? python3 readHBmap.py -hbm hbmap.xpm -hbn rg.ndx -f...

26 January 2022 7,142 5 View

Is there any other alternatives to Ni foam in supercapacitor ?

30 December 2017 2,247 7 View

Which surfactant is suitable for dispersing metal particles in chloroform or DI water solvent

I want no agglomeration of metal particles in the PLA- chlorofrom solvent solution. I am using solvent casting method to make the composite and finding the composite is not electrically...

25 July 2017 5,034 3 View

PLA/Fe(<10μm) composite is dissolved in Chloroform. Then sonicated for 10 mins after degassing for 5mins. I did not get a conductive composite. Why?

After PLA is dissolved in the chloroform, Fe(

25 June 2017 6,054 2 View

How to generate a incommensurate Supercell POSCAR for VASP?

Hello All, I have a 1x1x1 POSCAR, I need o make a 3 square root 3X1X1 supercell. I understand VESTA cannot make it, are there online tools available for generating this? I have attached my...

25 October 2016 8,510 4 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How to perform immunofluorescence on suspension cells?

Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...

20 July 2024 3,797 2 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Is perfusion required to stain brain tissue with every method?

I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...

14 July 2024 177 4 View

Does anybody have Anti-Claudin8 and Anti-Claudin2 Primary conjugated Ab (Target Species: Human)?

I need Anti-Claudin8 Primary and Anti-Claudin2 conjugated Ab for my immunofluorescence experiment. If anybody has worked on it. Please share the accession number/details.

14 July 2024 485 0 View

What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized?

What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized - I have few of them listed and can be updated in this...

13 July 2024 2,431 2 View

The complex interactions between oral microbiota and oral mucosal immunoregulation: implications for oral health and disease development?

This research question explores the complex interactions between oral microbes and oral mucosal immune regulation. The diversity and function of oral microbes have important impacts on oral health...

09 July 2024 7,883 0 View

Can I use BSA for a Histological Blocking Solution?

Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...

07 July 2024 7,967 3 View

Krish Kizhatil

Vasily, thanks for the info. I am using the BV13 clone from eBiosciences. I need an alternative. Krish

Xiang-Bin Wang

i have anti-human cadherin mAb in hand. DO you want to try it.

Hai T Yuan

Hi Vasily, Have you used BV13 by IF on mouse primary ECs. Can you share a IF image of BV13 on mouse primary endothelial cells if you have? We tried to use BV13, did not work in our hands for IF for cultured ECs, although it worked for mouse tisue sections. Any comments or suggestion?