Should a drug in liquid form (Geraniol) be first dissolved in solvent (DMSO/Ethanol) then diluted in media or directly added to media to make different concentrations for an MTT assay? Geraniol directly used in media is showing no effect .
Shishir Mishra
First approach is how i am doing it, i am using DMSO. For a different drug although.
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Heshu Sulaiman Rahman
Dear Kishore, do not use water for dissolving. Straightly dissolve it in DMSO, then try MTT, hope it works with you. TQ
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Mirza Muhammad Fahd Qadir
Chandra dissolve your drug in DMSO you might want to set up a MTT assay with different concentrations of DMSO as well, but first just use DMSO.
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Chandra Kishore
Drug is in liquid form then what is the need to dissolve in a solvent.why can't make different conc in media directly
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