I would like to purify RNA from lentiviral particles produced in Hek293's supernatent. Any protocol to suggest me ? Any advice ?
Thank you in advance :)
Hi,
you should be able to use any RNA virus extraction kit as the lentiviral vectors are developed based on HIV.
Good luck
Sven
Hi. Dr . Aurélie Delannoy
i suggest you that
1- Production of lentiviral vectors
Article Production of lentiviral vectors
2- Lentiviral Vector Production and Cell Transduction
https://www.invivogen.com/sites/default/files/invivogen/old/docs/Insight_201004.pdf?PHPSESSID=a81552686acd6275994a013320f5602c
3- Process development of lentiviral vector expression, purification and formulation for gene therapy applications
http://discovery.ucl.ac.uk/1485725/1/Nilsson_Thesis_Sara%20Nilsson_21Mar2016_post-corrections.pdf
and you can use any RNA virus extraction kit as the lentiviral vectors are developed based on HIV as Dr. Sven Reister suggested
good luck
Thank you all for your advices !
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