Genome database sequnces

More Rahul Shelke's questions See All

What are the roles of both Monetry and Fiscal Policy coordination in optimising the Macroeconomic outcomes in the economy ?

These days policy coordination is becoming necesssary on different front. In India also it is the same case. Monetary and Fiscal policy interface is important in order to address the...

30 July 2024 2,104 6 View

Can we fit a gaussian Fit on a LIBS Peak ?

Though much literature suggests fitting a Voigt profile on a LIBS peak, is it also possible to fit a Gaussian profile on the LIBS peak? I have 204 spectra files, and I am building a pipeline for...

23 July 2024 6,127 1 View

Can the MDFT mental health care model help reduce drug addiction?

This question might come up in my research on "Frustration, Fantasy, and Drug Addiction," where I'm also trying to figure out how to overcome mental health issues without turning to drugs.

30 June 2024 5,936 0 View

What is the effect of redshift on the evolution of an AGN?

AGN (Active Galactic Nuclei) are high energy galaxies powered by supermassive blackholes. The evolution of a galaxy is characterized by parameters such as luminosity and redshift. The redshift is...

25 June 2024 3,782 2 View

Which smoothening model is acceptable for processing of circular dichroism spectra?

For smoothening of noisy CD spectra, several fitting models are available in Jasco spectra manager software, like Savitzky Golay, binomial, means movement and adaptive smoothening. Each one asks...

16 June 2024 3,997 1 View

I am carrying out MTT Assay using HeLa cells but the absorbance values are really less, can anyone please help?

I have seeded HeLa cells in a calculation of 10000 cells per well in a 96 well plate. Then I went for treatment in the next day. Then at last, I added 7ul of 5mg/ml solution of MTT reagent into...

12 June 2024 1,460 3 View

Can we use LIBS Peak - Ti 441.492 for the Univarate Analysis ?

I have 17 different steel samples, in which i identified a peak of Ti 441.492. But , if you look closely at photo, while some some peak are at 441.492 , some are at the slight offset. eg 441.490...

10 June 2024 8,934 2 View

Challenges in Univariate Analysis for Stainless Steel Samples using Laser Induced Breakdown Spectroscopy?

Respected Members , I have a 17 different steel sample references , with varying concentration for Minor Elements being Mo , Ni , Mn ,Si , Cr etc , i am trying to build a calibration curve with...

10 June 2024 993 0 View

Is radio emission thermally or non thermally dominated?

Thermal emission is temperature dependent. Non-thermal emission is not temperature dependent such as synchrotron emission.

29 May 2024 5,155 2 View

Normalization in LIBS Spectra ?

Hello Everyone ! I have a samples a satinless steel , I performed the LIBS Measurement with hLIBS (SciAps Z-300). Total of 12 measurements on different locations were done on the same sample...

09 May 2024 7,959 6 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

How to use NCBI datasets ?

I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...

20 July 2024 1,339 2 View

Enquire about the calculation of percentage of reads mapped to the reference sequence?

Hello researchers, I have sequenced the A549 cell line using RNA sequencing approach and then I have it against the human genome reference sequence. Would I expect a 100% mapping percent? I got...

20 July 2024 9,705 0 View

Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

Where can I get PCR Primers for COI barcoding of birds?

We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...

16 July 2024 118 2 View

What is the most effective method for knocking out a gene in Candida albicans?

I have attempted various methods, including homozygous gene disruption and CRISPR-Cas9, but have not achieved the desired results. It appears that the gene of interest is situated in a...

15 July 2024 3,977 1 View

Abhijeet Singh

You might need to reconsider your the definition of genome and ploidy in organisms. But, actually, complete genome is the genetic material in an organism, nuclear or extra-nuclear. And ploidy works with just multiples of nuclear material.

Rahul Shelke

Suppose, haploid genome size of one plant is 1200 mbp and diploid is 2400 mbp.

In this case, whether genome database will contain 1200 mbp or 2400 mbp sequences.

1200 mbp + extranuclear genetic material