As a microbial ecologist studying novel environments where many microorganisms are difficult to culture I am curious about emerging methods that can improve the gap between genotype and phenotype (especially morphological information). I am interested to know how DNA sequencing through microscopy might be used to sequence DNA in situ especially in the case of organisms that may be difficult to identify through metagenome assembled genomes (MAGs) or single-cell genomes (SCGs). How far away is this technology? What are the obstacles to move from sequencing DNA extracts to in vivo sequencing using microscopic techniques? Do others share the view that this would be a particularly useful way to pair morphological information with genome data?
(Here are some resources for those interested in this discussion)
Although these papers are talking about the optic sequencing, however, neither of them are talking about the in-vivo sequencing.
For DNA sequencing, you need to get the DNA out of the cell. It is not a linear strand which is present in the cell so that it can be focused and can be sequenced. DNA is present in a complex structure.
So, what you are asking in your question is not possible. At least in next 10-20 years.
- Badges
- Science method