Could expired BSA a problem for tissue culture?

Hi, I want to ask if for you using expired BSA powder (expired in 2014 but always in the fridge and protected from light) could be a problem for my bovine primary follicles culture in situ (so...

01 December 2020 8,650 2 View

Neyman - Scott Cluster Process: are these nonsensical parameter values ?

I am trying to fit a cluster model in spatstat in R using the locations of archaeological sites. The resulting parameters values are: trend: 2.514429e-06 kappa: 1.606992e-08 scale: 481.5851 mu:...

18 November 2020 2,993 3 View

Flow cytometry from harvested non-uniform tissue?

Hi everyone! I want to identify by flow cytometry the immune cells found in the brain with a localized injury. My hypotesis is that the injury will attract more/different immune cells respect to...

27 October 2020 748 2 View

What do you use to avoid insect collection infestation?

At the museum we are currently dismissing Paradichlorobenzene and Naphtalene. we are looking for other effective chemical or similar.

21 October 2020 9,553 11 View

Issue with plasmid cloning?

22 August 2020 1,687 15 View

Is there any commercial or open source DIC (2D or 3D) software which is capable of working on field photographs or satellite images?

Is there any commercial or open-source DIC (2D or 3D) software which is capable of working on field photographs of terrain or satellite images for deformation and strain analysis in geophysical...

11 August 2020 4,216 3 View

How to perform in silico peptide cyclization reactions by ChemAxon Reactor?

24 April 2020 9,398 1 View

Does the EGFP epression in a BAC-EGFP mouse correlate with the amount of promoter gene expression?

Hi, I am analyzing immunostainings of 5-HT2AR-EGFP transgenic mice (Htr2a Gene and 5-HT2A Receptor Expression in the Cerebral Co... ) and I have a doubt regarding the conclusion I can draw about...

21 July 2019 209 1 View

Is it possible to "paste" two functions in COMSOL?

Hi everyone, here is the description of the problem. I have a rectangle with some circular holes in it, the inner part of the domain (denoted with D) is defined by the difference between the...

07 July 2019 1,635 3 View

How to avoid for order effects in an experiment with 20 participants and one task with 12 trials?

Hey, we are studying the relationship between trait anxiety and spatial navigation. We have 20 participants and one navigation task with 12 trials. How to avoid for order effects among the trials?...

07 April 2019 9,479 3 View

Triggering feeding of beetles / how to make them hungry?

Hey, I am working with Psylliodes chrysocephala feeding on oilseed rape. In feeding trials I often have the problem that a huge portion of the beetles do not feed at all. In clip cages I observed...

19 January 2021 9,851 6 View

Detection of insect eggs in stored and processed products?

Currently, in order to identify insect eggs in stored or processed products (such as contaminated flour or wafer samples), staining was performed based on the method mentioned in the below...

15 January 2021 6,362 5 View

Help with insect identification?

Could someone help me identify the insect in this picture?

31 December 2020 2,109 5 View

What are the best methods for clearning and mounting old Insects?

Could anyone suggest appropriate methods for positioning of legs, wings and antennae of dry Beetles. Methods for cleaning of their body as well. Insects are small in size about 2mm. I will be...

27 December 2020 2,544 7 View

Can we DNA barcode dry Museum insect specimens?

I am an student of entomological field, I am interested to work on DNA barconding of beetles, I have some fresh collection and some Museum specimens as well. I am not much clear that whether...

30 November 2020 7,248 14 View

What are the main taxa of above ground arthropods in a silvopasture system in the tropics of Latin America?

Beside dung beetles, I would like to learn more about the diversity of arthropods in silvopasture systems where livestock are kept on rotation. Also what about arthropods diversity in similar...

31 October 2020 6,632 1 View

What species of beetle (Insecta: Coleoptera) is this?

Dear all, I would like to ask for help in identifying this species of beetle from Sarawak (Malaysian Borneo). The total length is approximately 3 mm. It is sampled from a peat swamp forest near...

17 September 2020 9,636 10 View

Do you know this coccinelid beetle spp?

Dear all, I have found this coccinellid beetle specimen on a survey and did not able to identify the spp. Size and shape very much similar to Micraspis discolour. But having black colour big...

16 September 2020 9,965 6 View

Is Cucujus clavipes (Coleoptera: Cucujidae) legaly protected or included in the Red Data List/Book in any state of the USA or Canada?

Most (especially European and Asiatic) Cucujus species (Coleoptera: Cucujidae) are known as rare or threatened or even endangered. Is the same situation in case of the American Cucujus clavipes...

15 September 2020 9,190 4 View

Will Cypridina Luciferase (CLuc) Catalyze Beetle Luciferin?

Hello, I am using a lentivirus encoding CLuc to transduce target cells. I want to then lyse the cells in the presence of luciferin and measure luminescence. The kit which I had hoped to use...

15 September 2020 6,664 2 View