Frozen Whole Blood RNA extraction, which is the best kit?

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Andrew Octavian Sasmita

I do agree that QIAGEN kits are top notch, but I reckon the protocol for RNA extraction using QIAGEN kits from frozen whole blood is rather different than the conventional RNA extraction from fresh blood samples. You might want to treat your frozen blood sample with QIAzol until the sample thaws and comes into contact with QIAzol before proceeding with the RNeasy Lipid Tissue protocol (https://www.qiagen.com/us/shop/sample-technologies/rna/total-rna/rneasy-lipid-tissue-mini-kit) as recommended by suppliers.

Hassan Nima

Agree with @ Andrew Octavian Sasmita

Regards

Fatemeh Ranjbarnejad

Thanks everybody.