I need an efficient amniocyte culture, harvesting and G-banding protocol providing higher rate of metaphasic cell, higher quality of bands and monochromatid chromosomes.
Please could someone help me to solve this problem?
I need to extract total RNA from frozen whole blood samples in order to perform Real-time RT-PCR. Does anyone know which is the best kit to obtain high purity RNA suitable for this purpose? The...
31 December 2017 6,534 3 View
How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...
24 February 2021 166 6 View
I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...
23 February 2021 7,842 1 View
I am planning to hybradize Q-Learning algorthm with Genetic algorithm at the population initialization stage, crossover stage and mutation stage. The problem under consideration is a...
07 February 2021 3,123 8 View
We have been color banding American Kestrels using A-craft alpha-numeric color bands since ~2013 and would like to test other options. I am curious if any other researchers have experience using...
01 February 2021 6,786 3 View
I have a DNA sequence which has predictability to form G quadruplex . I need a software to generate a pdb of it which will form G quadruplex. I am using open Babel but I am not able to set the...
29 January 2021 6,190 4 View
I must put 50 kb plasmid into ecoli strain. I think 50 kb is too big for heat shock with ecoli. Is that correct? Will electroporation work? Or do I need to use cos sites and lambda phage...
22 January 2021 8,612 3 View
I'm looking for a bioinformatics website to get the chromosomal location of a radish gene id list, but in one shot instead of doing it gene by gene. Is there a way to do this? Thanks
17 January 2021 8,810 3 View
When conducting a simple linear regression analysis with one categorical predictor variable and power projected at .80, G*Power suggests a minimum sample size of n = 55. Now, assume that the...
10 January 2021 6,317 6 View
Hello, I am trying to mark cells undergoing proliferation in the brain of adult zebrafish (Danio rerio). I did immunohistochemistry on sections using the Anti-PCNA antibody [PC10] (ab29) with...
14 December 2020 4,989 1 View
just wanna check about with other cytogeneticist opinion is it t(1;16)(q1;q1)? and if so what do you know about such a mutation? P.S I added some karyotypes to be viewed
02 December 2020 6,827 1 View