Does anyone have experience with the preparation of human amniocyte karyotype?

Fatemeh Ranjbarnejad @Fatemeh_Ranjbarnejad2

04 October 2022 0 307 Report

I need an efficient amniocyte culture, harvesting and G-banding protocol providing higher rate of metaphasic cell, higher quality of bands and monochromatid chromosomes.

Please could someone help me to solve this problem?

Badges
Science topic

More Fatemeh Ranjbarnejad's questions See All

I have a problem with real time PCR, please help me if possible?

I have a problem with real time PCR, please help me if possible I was extracted total RNA of human peripheral blood and OD260/280 was in range of 1.6-2. cDNA was specifically synthesized with a...

21 July 2023 2,037 21 View

Frozen Whole Blood RNA extraction, which is the best kit?

I need to extract total RNA from frozen whole blood samples in order to perform Real-time RT-PCR. Does anyone know which is the best kit to obtain high purity RNA suitable for this purpose? The...

22 January 2018 3,703 1 View

Frozen Whole Blood RNA extraction, which is the best kit?

I need to extract total RNA from frozen whole blood samples in order to perform Real-time RT-PCR. Does anyone know which is the best kit to obtain high purity RNA suitable for this purpose? The...

31 December 2017 6,614 3 View

How to conduct a sensitivity power analysis for Kendall's Tau?

Is there a straightforward way to conduct a sensitivity power analysis for a Kendall's Tau correlation? I was considering using the sensitivity setting and "Correlation: point biserial model" test...

28 July 2024 6,133 8 View

How can I calculate minimal sample size using G Power?

I am conducting a study aimed at exploring the correlation between introversion and social phobia among Lebanese adults. I need to calculate the minimal sample size using G Power, but I'm not sure...

19 July 2024 6,048 1 View

How many established theories of macroevolution?

There are seven (7) established theories of macroevolution viz. i) Chromosomal speciation theory, ii) Shifting balance theory, iii) Punctuated equilibrium theory, iv) Allopatric speciation theory,...

10 July 2024 9,679 1 View

How to interpret Sensitivity Power Analysis for 2x2x2 repeated measures ANOVA?

I want to calculate the sensitivity using G*Power. If I have understood correctly G*Power gives me the detectable effect size for three-way interactions if I enter the number of groups (meaning...

08 July 2024 8,653 2 View

Chromosomal spreads are shrunken after fluorescence in situ hybridization (FISH), why?

Dear colleagues, We are trying to do FISH on mice PDEC metaphase spreads. We use homemade biotin labelled probes, synthetized using a nick translation kit. For hybridization, initially we were...

08 July 2024 8,687 3 View

How to prevent microbubbles during immunofluorescence imaging?

Hi ResearchGate community, how are you? I need to request your help to mitigate the generation of microbubbles detected during the slide preparation of sample metaphase chromosomal spreads. I...

01 July 2024 7,694 4 View

Why the secondary constriction region of chromosome i.e. known as NOR, is non-staining region?

Silver staining process is used to stain this region, why?

12 June 2024 4,517 1 View

Question regarding G*Power and whether I should use R-squared increase or R-squared deviation from zero?

So let's say I wanted to do a research with this topic: "Factors affecting QOL of beta-thalassemic patients" Study Design: Cross Sectional Number of Independent Variables: 21 Dependent...

10 June 2024 2,706 6 View

How many chromosomes have Greek plants Prometheum tymphaeum and Sedum tristriatum?

Contandriopoulos, Quézel et Zaffran claimed that both 2n=16 whereas 't Hart claimed tymphaeum 2n=14 tristriatum 2n=22, 2n=44

29 May 2024 3,952 1 View

Has anyone used Dinitroaniline herbicides to double the chromosomes of haploid corn?

Greetings! Has anyone used Dinitroaniline herbicides to double the chromosomes of haploid corn? If did, which one and the details, please. Thank you very much!

29 May 2024 9,836 1 View