I need an efficient amniocyte culture, harvesting and G-banding protocol providing higher rate of metaphasic cell, higher quality of bands and monochromatid chromosomes.
Please could someone help me to solve this problem?
I have a problem with real time PCR, please help me if possible I was extracted total RNA of human peripheral blood and OD260/280 was in range of 1.6-2. cDNA was specifically synthesized with a...
21 July 2023 2,037 21 View
I need to extract total RNA from frozen whole blood samples in order to perform Real-time RT-PCR. Does anyone know which is the best kit to obtain high purity RNA suitable for this purpose? The...
22 January 2018 3,703 1 View
31 December 2017 6,614 3 View
Is there a straightforward way to conduct a sensitivity power analysis for a Kendall's Tau correlation? I was considering using the sensitivity setting and "Correlation: point biserial model" test...
28 July 2024 6,133 8 View
I am conducting a study aimed at exploring the correlation between introversion and social phobia among Lebanese adults. I need to calculate the minimal sample size using G Power, but I'm not sure...
19 July 2024 6,048 1 View
There are seven (7) established theories of macroevolution viz. i) Chromosomal speciation theory, ii) Shifting balance theory, iii) Punctuated equilibrium theory, iv) Allopatric speciation theory,...
10 July 2024 9,679 1 View
I want to calculate the sensitivity using G*Power. If I have understood correctly G*Power gives me the detectable effect size for three-way interactions if I enter the number of groups (meaning...
08 July 2024 8,653 2 View
Dear colleagues, We are trying to do FISH on mice PDEC metaphase spreads. We use homemade biotin labelled probes, synthetized using a nick translation kit. For hybridization, initially we were...
08 July 2024 8,687 3 View
Hi ResearchGate community, how are you? I need to request your help to mitigate the generation of microbubbles detected during the slide preparation of sample metaphase chromosomal spreads. I...
01 July 2024 7,694 4 View
Silver staining process is used to stain this region, why?
12 June 2024 4,517 1 View
So let's say I wanted to do a research with this topic: "Factors affecting QOL of beta-thalassemic patients" Study Design: Cross Sectional Number of Independent Variables: 21 Dependent...
10 June 2024 2,706 6 View
Contandriopoulos, Quézel et Zaffran claimed that both 2n=16 whereas 't Hart claimed tymphaeum 2n=14 tristriatum 2n=22, 2n=44
29 May 2024 3,952 1 View
Greetings! Has anyone used Dinitroaniline herbicides to double the chromosomes of haploid corn? If did, which one and the details, please. Thank you very much!
29 May 2024 9,836 1 View