Multiple sgRNA cloning into pX333 plasmid using BsaI and BbsI restriction enzyme site?

Currently, I am using a plasmid containing two gRNA cloning site. The restriction enzymes used for cloning gRNAs into this plasmid, pX333, are BsaI and BbsI. I digested the pX333 plasmid using...

09 October 2019 2,743 6 View

How do I design primers specific to transfected plasmid and not genomic DNA?

I recently introduced a Lenti-Puro Guide vector containing a specific sgRNA sequence into a cell line. I would like to sequence the ddCas9-sgRNA cell lines to confirm that each sgRNA is accurate.

01 February 2019 5,376 3 View

Has anyone uses a 4in1 cholesterol tester on research mice?

Hi all, Has anyone used a 4in1 cholesterol tester on research mice? We have used the One Touch handheld glucose monitoring system to measure glucose on high fed diet fed mice. However, has anyone...

09 October 2016 3,866 2 View

Does anyone know properly analyze CLS stuctures? What features should I look for (eg. small adipocytes surrounded by cells)?

The adipose have been sectioned and stained with H and E. Of couse F4/80 is required, however I would to crudely analyze these sections.

01 February 2016 589 2 View

Should I serum starve primary hepatocytes if I wish to probe for pAMPK using westen blotting?

I am using stimulated primary hepatocytes to detect alterations in AMPK activity. However, a paper published that serum starvation can increase AMPK activity in different cell types. Although,...

07 August 2015 7,940 3 View

Does anyone know of easily transfected mouse cell lines?

I would like to assess the editing efficiency of designed guide RNAs by transfection prior to establishing a stable KO cell line (via lentiviral infection). HEK293T cells are very efficient when...

01 January 1970 6,434 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Can I get the design of circular cylinder to fix thermocouple on the surface and cartridge heater in core ?

Respected Scientific Community. I'm interested to carry out experimental studies on a circular cylinder. But even after doing literature review I unable to get the exact design of the cylinder...

10 February 2021 6,221 1 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

What is the difference between master and doctoral degree?

Hello there.. I've recently submitted my master thesis in marketing. As a reseacher, I now know that the purpose of a master degree is to know academic research tools and how to do and write an...

09 February 2021 6,692 9 View

Could you please suggest 3D opensource plotting tool for spheres, cylinders and other shapes?

I will be plotting multiple such shapes. Hence, I am looking for an interactive slice through option to see if there are any overlaps. Thanks,

04 February 2021 861 6 View

Where can I get these scientific reports?

Friend, can I ask you a question? Please, can you help me with the search for these pdf files that are scientific publications made in China? 1. Fengxing, Z., Zhizhen, G., Peiheng, C., Jinhua, M.,...

02 February 2021 6,261 5 View