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Questions related from Joselyn Allen
Currently, I am using a plasmid containing two gRNA cloning site. The restriction enzymes used for cloning gRNAs into this plasmid, pX333, are BsaI and BbsI. I digested the pX333 plasmid using...
10 October 2019 2,867 6 View
I recently introduced a Lenti-Puro Guide vector containing a specific sgRNA sequence into a cell line. I would like to sequence the ddCas9-sgRNA cell lines to confirm that each sgRNA is accurate.
02 February 2019 5,415 3 View
Hi all, Has anyone used a 4in1 cholesterol tester on research mice? We have used the One Touch handheld glucose monitoring system to measure glucose on high fed diet fed mice. However, has anyone...
10 October 2016 3,914 2 View
The adipose have been sectioned and stained with H and E. Of couse F4/80 is required, however I would to crudely analyze these sections.
02 February 2016 634 2 View
I am using stimulated primary hepatocytes to detect alterations in AMPK activity. However, a paper published that serum starvation can increase AMPK activity in different cell types. Although,...
08 August 2015 7,990 3 View
Are you in need of a scientific illustrator for creating illustrations (cellular and molecular mechanisms, structures, etc) to compliment a primary paper or review article? If so, please let me...
08 August 2015 5,459 0 View
I would like to assess the editing efficiency of designed guide RNAs by transfection prior to establishing a stable KO cell line (via lentiviral infection). HEK293T cells are very efficient when...
01 January 1970 6,479 2 View
