What is the best technique to identify native bacteria groups present in soil?

I am lay in this subject. Is DNA sequencing the best way to identify the different types of soil bacteria present in soil samples? Ideally, I'd like to find a technique that allows me to identify...

29 September 2020 4,609 9 View

Do you have any suggestion about constrained estimation of mixed models using lme4 package of R?

I would like to use the lmer function of the lme4 package to estimate the random part of the model given the coefficients of the fixed part (which have been estimated in a previous stage using...

24 February 2020 1,731 5 View

Can I use Nanostring microRNA count date (normalized or raw data) to measure relative gene expression?

Can I use Nanostring microRNA count data (normalized or raw data) to measure relative gene expression by comparing it with an endogenous control (mRNA) expression?

25 November 2019 277 2 View

ERT Optimisation protocol sequence software-code for 3D unconventional electrode arrays?

Anyone know about any freely available software or code that can optimise protocol sequences for 3D unconventional electrodes setups, including borehole electrodes? The closest research I found...

15 November 2019 7,802 3 View

How can I calculate the concentration of 30nM miRNA from a 5nMol stock?

I have a transfection experiment to be done. The stock miRNA is 5nMol. The manufacturer recommends adding 75uL of sterile water, giving me a concentration of 66.67 uM. How can I have a final...

05 May 2019 4,141 7 View

Do we have cell culture contamination?

We are experienced lately some problems with primary cells. We could see (even in 10x magnification) tiny bright particles around the addhered cells. There is no macroscopic sign of...

14 December 2016 7,464 11 View

Could anyone help me with PCR to Mycoplasma detection?

I'm tryint to standardize a PCR to mycoplasma detection of dna from cell culture. I'm testing samples positive for mycoplasma and negative as controls. I found a weak band unspecific slightly...

29 June 2016 7,818 3 View

Low caspase activity in heavy metal lethal concentration 50 (LC50). What does that mean?

02 June 2016 1,480 3 View

Can anyone help me to calculate the NP ratio considering that I have mass per phosphate of 13,300 g/mol and mass per amino of 43 gm/mol?

Can anyone help me to calculate the NP ratio considering that I have mass per phosphate of 13,300 g/mol and mass per amino of 43 gm/mol?

22 May 2016 9,403 1 View

How do I read the results of an ANOVA (2 independent factors) that instead of F reason, I got a Z? What is this Z?

I ran a 2 independent factors ANOVA in SPSS and got a Z score instead of F. Is that related to different sample sizes in different conditions? Do I need to even the sizes of the samples before...

08 March 2016 3,813 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View