For those of you who immunize with sheep red blood cells, can you freeze the RBC in 30% w/v dextran for later use? Does this impact the antigenicity?

Maureen A Cox @Maureen-Cox

19 October 2013 1 8K Report

I am trying to look at germinal center B cells and Tfh with my undergraduate student, hence no infectious work.

Daniel Byrd

Not sure about dextran, but freezing human RBCs in 10% DMSO causes the vast majority to lyse after thawing.

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