I am trying to look at germinal center B cells and Tfh with my undergraduate student, hence no infectious work.
Not sure about dextran, but freezing human RBCs in 10% DMSO causes the vast majority to lyse after thawing.
So I understand that essential oils are formed by steam distillation, and contain volatile, usually aromatic, compounds. Extracts are formed by immersion of plant matter in a solvent, and are...
12 May 2020 2,119 5 View
I am writing an article on this.
20 November 2017 5,867 2 View
In the past we grew cells on uncoated coverslips to monitor NF-kB activation via immunofluorescence. Recently we started to use this method again, but switched to poly-D-lysine coated coverslips...
15 November 2017 7,870 2 View
I'm examining a moderated-moderation model (IV = marital conflict; Moderator 1 = emotion regulation; Moderator 2 = Marital Status; DV = Marital Satisfaction). I used SEM to run two separate...
08 September 2017 5,537 0 View
Hello all, I have the task of analysing a smallRNA data set with a focus on microRNAs in tomato. The task (at least sounds) simple: how many known and novel microRNAs are there in our samples and...
16 October 2016 1,500 2 View
17 May 2016 9,765 4 View
31 March 2016 3,442 4 View
I am looking to detect my transcript of interest in the brain ring gland complex in polysome fractions and I am wondering what is the minimum amount of dissections I will need to do. Thanks all!
04 March 2015 510 3 View
I am new to Abaqus and experience some difficulties with performing a high cycle fatigue analysis on a stent strut. We would like to apply an internal displacement caused by the pulsation of the...
16 April 2014 4,984 2 View
I am going to purchase a 384 well qPCR system (either BioRad or ABI) and would like to get a liquid handling robotics station to go with it. If anyone has purchased a setup up like this and has...
30 May 2013 1,351 7 View
I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...
03 March 2021 1,931 5 View
I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why
02 March 2021 1,388 3 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...
01 March 2021 9,332 6 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...
26 February 2021 3,526 3 View