Hello everyone,

I am working on some set of genes of Plasmodium parasite and wanted to extract and analyze their gene expression data on wild type parasite and mutant ones. So I am designing the pipeline as follows:

1. Extraction of gene expression data either of RNAseq or microarray.

2. analying the data using Galaxy or iDep. (since I don't know any programming).

Queries:

1. How will I isolate the expression data for my set of genes from the pool of genes?

2. Can I use the RNAseq data or microarray data for Single cell RNA-seq analysis?

3. Is my pipeline is okay to proceed?