In a in vivo mouse project tamoxifen will be administered in subcutaneous implanted pellets.
What dosage and resorption time of subcutaneous tamoxifen pellets (e.g. 100 mg over 21 days = daily administration of 4,7 mg) do you recommend for sufficient Cre-ERT2 induction?
Thank you very much for your help!
Dear Mathias,
When it comes to Cre-Ert recombination, there is no real easy way. The recombination efficiency will highly rely on the promotor, the position in the DNA and the availability of the tamoxifen.
Thus, it is usually advisale that you actually do tests with some reporter mice (ROSA-bgalactosidase,ROSA-Tomato, commercialy available) anc combine that with Cre-immunostaining to actually have the real proportion of recombination and make your choice according to those results. On top of that, it is often (if not always) rquired for publication to have this typpe of results to show the specificty of the recombination.
Good uck!
Thibault
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