For Real Time PCR we are using bacteria cDNA what is the criteria for reference gene?

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What is the basis of selecting the bacteria/fungi from soil responsible for the biodegradation????

From soil we can isolate the bacetria/fungi by traditional serial dilution techniques. If anyone working on biodegaradtion than let me know about the screening parameters for microbes with respect...

03 April 2019 9,632 10 View

How methyl halide kill pest/insect and which types of pest it killed ? I want to know the exact mechanism in pest after methyl halide exposure????

Methyl halide pesticide cell biology/molecular biology in insect

03 April 2018 988 5 View

I want to do mutagenesis using insilico tools. What is the basis of mutant selection. How can I can prepare best mutation in enzyme active site?????

basis of selection of mutation on enzyme active sites.

03 April 2018 340 3 View

In Silico work how much fruitful in biological sciences??

Bioinformatics question

02 March 2018 7,812 10 View

Biochemical test of microorganisms have any importance as compare to recent molecular highthroughput tools...??

Biochemical test and molecular tools of microorganisms??

02 March 2018 8,707 0 View

I want to know about the Fuzzy analysis in Biodegradatiuon. Can anyone have Idea how to use fuzzy modeling for biodegradation of xenobiotics.????

I want to know about the Fuzzy analysis in Biodegradation. Can anyone have Idea how to use fuzzy modeling for biodegradation of xenobiotics.????

31 December 2017 9,714 1 View

How we can isolate the protein from the periplasm of gram negative bacteria???

Protein isolation

04 May 2017 8,822 2 View

How much error can be possible during experiment ? statistically 0.1% or 0.5% accepted ?

statistical error

02 March 2017 5,684 0 View

How we will ensure the isolated proteome contains all types of proteins during particular stress conditions.?

Proteomics

02 March 2017 9,174 0 View

Any one have idea how to knockout gene from bacteria??

gene knockout

02 March 2017 5,793 0 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Jagna Chmielowska-Bąk

As far as I know, for reference gene you always choose a housekeeping gene which shows stable expression in all of yours experimental variants. It is also advisable to use at least two reference genes.

Pouriya Gharavi-Kouchebagh

Dear Pankaj,

As Jagna and Maria said I do not think something be unclear.

Maria articles was excellent.

Sincerely,

Gharavi

PANKAJ BHATT

Thankyou Maria de los Angeles Serrano and all