Can we use RNA-seq library for gene expression studies?

Since our RNA is less abundant, can we use the libraries prepared for RNA-seq to validate (qPCR)the results of RNA-seq.

12 November 2020 803 6 View

What is the difference between semantic role labelling and named entity recognition?

Natural language understanding

04 May 2019 7,442 9 View

Is there a software or Webpage where I can view a 3D anatomy map of the brain?

I want to look at all the various brain regions and also the ascending and descending tracts from the brain, and make sense of the information flow.

12 March 2019 1,778 5 View

How to calculate network performance using ns3?

I'm new to this network domain. But i want to learn this area thoroughly. So I'm currently dealing this subject. I'm learning ns-3 simulator. Please help me to know how to analyze the performance...

17 September 2018 3,246 3 View

Acyliminium reactivity versus enecarbamate?

Dear researchers, i kindly ask you to help me with the following aspect: in scheme 4 (of j.org. chem, vol.66,No.23, 2001) it is shown that nucleophile attacks on the acyliminium intermediate and...

08 February 2016 1,601 3 View

I am using HEC RAS to connect various ponds and lakes in the city and perform flood inundation mapping. What is the suitable analysis?

I am using HEC RAS (Though it is a river analysis software) to an urban area to connect various ponds and lakes in the city and perform flood inundation mapping. What is the suitable...

01 February 2016 4,321 6 View

Why does K estimator fail to estimate Ka and Ks values for certain gene combinations?

12 February 2015 8,709 1 View

Can any one explain me the pixel recursion and how to calculate new disparity candidate using this?

Pixel recursion.

11 August 2014 2,495 2 View

Can anyone guide me about disparity estimation in stereo images using line-wise hybrid recursive matching algorithm?

Disparity estimation of stereo images. This involves several processing steps including pixel recursion, post processing, how to estimate disparity between two gray scale images using this algorithm.

11 August 2014 9,312 1 View

Why is a logarithmic current amplifier used instead of a linear one in scanning spreading resistance microscopy?

For measuring and amplifying current during a C-AFM scan, I'm aware that a linear current amplifier is generally used but in SSRM which is mostly used to get doping profiles in semiconductors, a...

11 April 2014 4,026 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View