Swathi Divakar

4 Questions 6 Answers 0 Followers

Questions related from Swathi Divakar

What is the difference between Qubit RNA Hs and Qubit RNA BR kit? Which is more appropriate for the tissue RNA?

The values given for the same sample vary between RNA HS (diluted and used, keeping in mind the sensitivity of the kit) and RNA BR.

26 July 2021 7,079 6 View

Why we are not normalizing with housekeeping gene in the NGS data analysis?

We normalize with the housekeeping gene in the qPCR anlaysis. But why we are not normalizing the NGS data with some housekeeping genes for differential gene expression?

20 November 2020 2,728 4 View

Can we use RNA-seq library for gene expression studies?

Since our RNA is less abundant, can we use the libraries prepared for RNA-seq to validate (qPCR)the results of RNA-seq.

13 November 2020 941 6 View

For primer efficiency calculation can we use positive control(in which gene is abundantly expressed) and perform the analysis?

My sample of interest is sperm RNA(less abundant and highly fragmented RNA). In that case, to determine the primer efficiency, can i use tissue samples (in which the gene is abundantly expressed)...

12 November 2020 925 3 View