IC50 value from this graph?

01 January 2021 6,848 4 View

What is the best Free/Open Source software for doing Taguchi and other design of experiments and associated meaningful analysis like ANOVA?

Also what are the different efficient DOE and Analysis methods w.r.to machining operation with a specially sintered tool?

03 August 2020 4,553 3 View

What is the current research scenario related to solid lubrication & solid lubricant based cutting tools?

What according to you should be researched/accessed with respect to WC based solid lubricant tools? What is your view with respect to future research in this area? (For e.g. Tribological study...

19 July 2020 3,232 6 View

Can I freely use figures from open access journals/articles by citing the article?

I am writing a review paper. In my paper, can I use figures from "open access" journals/articles etc provided I cite them properly? (i.e. without have to go for requesting permission for using)....

02 July 2020 9,363 15 View

Is it acceptable to add more conclusions in a conference paper that were not discussed due to shortage of space?

Hi, My research project had numerous results and because the conference paper is usually just 5-6 pages long, I could not discuss all my results in it. However, is it still possible to mention...

04 May 2020 527 5 View

Under what circumstances can we consider mass equivalent to weight and vice versa ?

Can mass be always considered equivalent to weight ?

17 September 2019 7,790 3 View

How to do mechanism study in photo-catalytic degradation of pollutant?

Dear Friends, I am currently doing some experiment where i prepared some nanomaterial and now trying to degrade dye molecule under visible and UV light condition. I need help regarding setting up...

21 May 2019 1,567 1 View

What would be right (to be ideal or Potential) concentration (ug/ml) of a plant crude drug extract on MCF-7 cell lines?

What would be right (to be ideal or Potential) concentration (ug/ml) of a plant crude drug extract at 50% inhibition (IC50) values against MCF-7 human breast cancer cell lines? I have red an...

14 May 2019 4,543 3 View

Any best resource (Online- paid/free anytihing) for learning Single Cell RNA-Seq Data Analysis online?

Hi community members ! I would be very happy to recieve advices from you. I have scRNA-Seq data and would like to start with the analysis part. I already went through QC and alignment process but...

10 March 2019 4,452 6 View

GPU accelerated implementation of 3D discrete Wavelet Transform?

I am looking for GPU accelerated implementation of 3D Discrete Wavelet transform but unfortunately I have not been able to find it yet online. Matlab's implementation does not uses GPU and is is...

31 January 2019 512 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

QTL, recombination frection?

22 February 2021 2,588 2 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?

19 February 2021 2,352 5 View