For MTT (cell viability assay), one should use round bottom or flat bottom 96-well plate?

More Debaashish Biswas's questions See All

Is there any cracked version available TuFLOW software? How to get tutorials of this software?

Is there any cracked version available TuFLOW software? How to get tutorials of this software . As i want to use for modified shallow water equation in urban flood modeling and simulation

06 July 2024 2,741 0 View

There are any alternative software Infoworks or Mike for urban flooding?

please suggest if there are any cracked versions of these software available.

04 July 2024 8,704 2 View

Which solvent and substrate is found to be best suited for deposition of electrodes with MXene ink?

Suppose we have dried MXene slurry (Ti3C2Tx), then the combination of which solvent (in which this dried MXene slurry powder will be mixed, subsequently forming the "ink") and corresponding...

13 June 2024 5,763 0 View

Do you think academia, particularly in the humanities and social sciences, is a continuum of semantic tricks?

Every concept feels like its predecessors stated it more precisely, with successors just adding context. It's rewording, but the understanding stays the same. For example: Structuralism, as...

10 June 2024 3,979 0 View

Can the aims outlined by a translator in the preface be understood as Skopos?

Skopos theory, developed by Hans Vermeer, is a functionalist approach to translation that emphasizes the purpose (Skopos) of a translation as the primary factor guiding the translation process....

02 June 2024 761 3 View

Which institute in India has Intelligent gravimetric analyzer for hydrogen gas storage?

16 May 2024 9,115 0 View

What type of validity is measured for questionnaires by Product Moment Pearson Correlation in SPSS?

Product Moment Pearson Correlation was done in SPSS for the validity test by correlating each item's questionnaire scores with the total score. What type of validity (content/ criterion/ construct...

27 April 2024 4,753 2 View

How can we induce drought stress in rice plants in vitro? What methods measure stress( MPa) accurately?

This question seeks to explore the techniques for inducing drought stress in rice plants in laboratory conditions and methods for accurately assessing the resulting MPa stress levels. It aims to...

17 March 2024 5,840 0 View

How to create a map of strip cultivation?

I want to create a map of specific cultivation, which is very narrow. It is not possible to identify in satellite image with 15 m resolution and also not possible to digitize on Google Earth Pro....

25 January 2024 2,818 2 View

Why there are traces of fluoride particles in my supernatant while synthesizing MXenes (Ti3C2Tx)?

I am trying to synthesize MXenes (Ti3C2Tx), using the MILD etching method (in-situ formation of HF by reaction of LiF + HCl). I am taking equal amounts of LiF powder (Sigma Aldrich, >99.99%...

24 January 2024 986 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View