For IHC, antigen retrieval solution should it be provided fresh?

Marzieh Eghtedardoost @Marzieh-Eghtedardoost

14 September 2015 1 5K Report

can I reuse antigen retrieval solution (citrat buffer) for 2-3 times in different IHC run? or once used?

In my experience, I reuse about five times. The results loos OK. But I don't know what will happened if buffer use more than fives times.

Badges
Science topic

Similar topics
Filing

More Marzieh Eghtedardoost's questions See All

How to dissolve Span 80 in mineral oil or liquid paraffin?

I want to work on microfluidic chip. Dispersed phase of alginate solution and continuous phase of Span 80 solution in combination with mineral oil or liquid paraffin. But I had a problem with the...

19 May 2024 1,790 7 View

Why we did not have clear bands with FluorChem protein simple imaging system?

we did not have clear bands with FluorChem protein simple imaging system .That is, the intensity of the bands are so high that the distance between the wells containing different samples are not...

26 January 2024 9,162 0 View

Should the new column from the subproblem always go into the base of the master problem in the next iteration?

In some iterations, when a new column is added to the master, it does not enter the base and a stop condition occurs, or a new column with a better objective function is found. is it correct? When...

20 November 2023 8,677 0 View

Can we consider incident rate ratio equal to hazard ratio in meta-analysis?

In a meta-analysis, there is just one study that reported the incident rate ratio, can I consider it as HR?

03 November 2023 1,845 4 View

Why my transduction efficiency doesn't exceed 10% with MOI=10 in Jurkat cells?

I am expressing two genes (CAR and a reporter) under EF-1a promoter in a lentiviral vector. I am using 2nd-generation lentiviral system (psPAX2 and VSV-G). My transfection efficiency in 293LTV...

24 October 2023 5,750 5 View

Whats the simplest method to measure OH radicals in a solution?

Hello everyone, I am currently involved in research focused on the electrochemical removal of synthetic wastewater as part of my project. I am seeking the simplest method to measure the OH...

04 October 2023 6,694 3 View

How to prevent the aggregation of positively charged nanoparticles in the aqueous environment?

I have made positively charged nanoparticles with Lecithin, Squalene, Tween 80, Span 85 and Dotap. But after using ultrasonic, the particles are aggregated and their size increases. How can...

10 September 2023 3,280 3 View

Hi, what is the problem?

I made cationic nanocarrier by lecithin, Squalen, tween 80, span 85 and DOTAP via melting method in 80 C, but nanoparticles had negative charge instead of positive charge. Does anyone know where...

04 August 2023 5,742 1 View

Can lipid nanoparticles larger than 200 nm pass through the 0.2 filter due to flexibility?

Hi, Can lipid nanoparticles larger than 200 nm pass through the 0.2 filter due to flexibility?

04 August 2023 2,727 2 View

What is the Maldi-tof technique?

What is the Maldi-tof technique? Is it necessary to perform Maldi-tof technique after protein purification?

16 June 2023 9,555 4 View

What exactly is RAG-LLM doing? Isn’t it data engineering?

What exactly is Retrieval Augmented Generation for Large Language Model doing? Isn’t it data engineering?

30 July 2024 7,376 3 View

May I know the exact Quartile of the journal- Advanced Engineering Materials (Wiley) for material science category?

In some data sources it has been grouped in Q1 and some shows it is Q2.

29 July 2024 4,227 2 View

I need the housing or real estate prices data since 1950 till date in India. Can anyone guide how can I get this data ?

I didn't find any data source for continuous time series since 1950. NHB Residex and RBI give data from 2013 and 2010 onwards. Please guide. Thanks !!!

27 July 2024 6,271 5 View

Do I need an antigen retrieval for a multiplex RNAscope experiment for adherent cells?

Hello! I planned to do an multiplex RNAscope fluorescent assay for cultured cells in a 96 well plate format according to the protocol provided by ACD:...

22 July 2024 3,334 0 View

Information is Stored by Context?

While living in Boston, which has a fine metro system, there were times that I would exit from underground at the wrong location, thereby expecting a particular visual scene only to be momentarily...

17 July 2024 3,237 10 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

My question is about errors in Ansys mechanical APDL? Data file file.db does not exist for RESUME?

I did a welding simulation using ANSYS MECHANICAL APDL. The solution was done, but I encountered this error in the output file (Data file file.db does not exist for RESUME). On the other hand, I...

11 July 2024 4,480 0 View

How can I avoid uneven IHC staining on free floating tissue from frozen human CNS sections (cryostat 50um thickness)?

Hello, I've been doing free floating IHC using a VAChT antibody with a 1:1k dilution (139 103 - Synaptic Systems). However, there has been a struggle to keep an even stain with my samples of...

06 July 2024 4,521 2 View

Is it recommended to use DNA LoBind tubes for protein extraction - and how, if at all, does it affect retrieval of proteins?

Hi, in our group we are trying to optimize a protein extraction protocol, and I've noticed DNA LoBind tube and DNA LoBind tips are being used. I wanted to know if it is ok to use DNA LoBind for...

30 June 2024 7,751 2 View

Can anyone recommend an antibody development course?

I would like to find a short course focused on the development of monoclonal antibodies covering the entire process, from the selection of antigen and carrier, to the purification of the final...

27 June 2024 5,618 1 View