Please share your working experiences and views with regard to the context. Have you ever noticed?? Your answer will help me understand why I can't replicate the results produced earlier with similar reagents/chemicals.
Well, I guess what comes out of a reaction mixture depends on what you put in it, don't you think? David E Booth
Hi David Eugene Booth,
Thank you for the kind response. As you have said, you may put in reagents for a particular reaction to take place. But the proper reaction occurs only when the added reagents possess the particular purity level and also reagent preparation in chemical industries differ. So, in this case I would love to hear your experiences working with chemicals/ reagents of varying quality and the results obtained. Thank you for the earlier response and you are welcome to answer more.
What you said was exactly what I meant. GIGO Why do you suppose Analytical Chemists use Analytical grade reagents?. Further, why do you suppose biochemical preps don't always work in different hands? Not everything is the same. I am surprised a chemist would ask this question. As an example try to prepare PhiX174 closed circular DNA by what is written in a paper. That is why biochemists have strict protocols for everything. Ciao
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